Treatment of Corneal Infections Utilizing an Ocular Wound Chamber.

Translational Vision Science & Technology
Jennifer S McDanielGina L Griffith

Abstract

To demonstrate that the ocular wound chamber (OWC) can be used for the treatment of bacterial keratitis (BK). A blepharotomy was performed on anesthetized, hairless guinea pigs to induce exposure keratopathy 72 hours before corneal wound creation and Pseudomonas aeruginosa inoculation. Twenty-four hours postinoculation, eyes were treated with an OWC filled with 500 µL 0.5% moxifloxacin hydrochloride ophthalmic solution (OWC), 10 µL 0.5% moxifloxacin hydrochloride drops (DROPS) four times daily, or not treated (NT). White light, fluorescein, and spectral domain optical coherence tomography (SD-OCT) images; ocular and periocular tissues samples for colony-forming units (CFU) quantification; and plasma samples were collected at 24 and 72 hours posttreatment. White light, fluorescein, and SD-OCT imaging suggests OWC-treated eyes are qualitatively healthier than those in DROPS or NT groups. At 24 hours, the median number of CFUs (interquartile range) measured was 0 (0-8750), 150,000 (106,750-181,250), and 8750 (2525-16,000) CFU/mL for OWC, NT, and DROPS, respectively. While 100% of NT and DROPS animals remained infected at 24 hours, only 25% of OWC-treated animals showed infection. Skin samples at 24 hours showed infection percentag...Continue Reading

References

Jan 1, 1992·The Journal of Surgical Research·K BreuingD R Miller
Mar 20, 1998·The British Journal of Ophthalmology·M SrinivasanJ P Whitcher
Jun 19, 2003·The British Journal of Ophthalmology·B H Jeng, S D McLeod
Jun 19, 2003·The British Journal of Ophthalmology·T BourcierL Laroche
Nov 14, 2006·Advanced Drug Delivery Reviews·Arto Urtti
Feb 5, 2008·Cornea·Matthew GreenFiona Stapleton
Nov 27, 2009·BMC Ophthalmology·Andrew F Smith, Curtis Waycaster
May 4, 2010·The AAPS Journal·Ripal GaudanaAshim K Mitra
Nov 26, 2010·Seminars in Ophthalmology·Dimosthenis MantopoulosPedram Hamrah
Dec 3, 2011·The British Journal of Ophthalmology·Donatella Pascolini, Silvio Paolo Mariotti
Jan 17, 2013·The Journal of Craniofacial Surgery·Johan P E JunkerElof Eriksson
Mar 4, 2014·Advances in Wound Care·Johan P E JunkerElof Eriksson
Oct 4, 2014·Saudi Journal of Ophthalmology : Official Journal of the Saudi Ophthalmological Society·Lee R Katzman, Bennie H Jeng
Dec 3, 2014·Progress in Retinal and Eye Research·A J BronF V Bright
Jan 23, 2016·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·David M TsaiE J Caterson
Oct 27, 2016·Plastic and Reconstructive Surgery·Lauren Tracy DalyEdward J Caterson
Nov 1, 2016·Drug Delivery and Translational Research·Vibhuti AgrahariAshim K Mitra
Sep 25, 2017·Ophthalmology·Ariana AustinJennifer Rose-Nussbaumer
Nov 7, 2019·Saudi Journal of Ophthalmology : Official Journal of the Saudi Ophthalmological Society·Hind M Alkatan, Rakan S Al-Essa
Nov 22, 2019·Journal of Medical Microbiology·Yasmin HilliamCraig Winstanley

❮ Previous
Next ❯

Citations

Nov 18, 2020·Translational Vision Science & Technology·Patrick M McNutt, Rajiv R Mohan
Feb 18, 2021·Experimental Eye Research·Gina L GriffithJennifer S McDaniel

❮ Previous
Next ❯

Methods Mentioned

BETA
scrapings

Software Mentioned

ImageJ
SAS

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

© 2021 Meta ULC. All rights reserved