Treatment of Cutaneous Ulcers with Multilayered Mixed Sheets of Autologous Fibroblasts and Peripheral Blood Mononuclear Cells

Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology
Takahiro MizoguchiKimikazu Hamano

Abstract

We have developed a mixed-cell sheet consisting of autologous fibroblasts and peripheral blood mononuclear cells with a high potency for angiogenesis and wound healing against refractory cutaneous ulcers in mouse and rabbit models. To increase the effectiveness of the mixed sheet, we developed a multilayered mixed sheet. We assessed the therapeutic effects of multilayered sheets on cutaneous ulcers in mice. Growth factors and chemokines were assessed by enzyme-linked immunosorbent assay. Angiogenesis and fibroblast migration were measured by using tube formation and migration assays. Wound healing rate of cutaneous ulcers was evaluated in mice with diabetes mellitus. The concentration of secreted vascular endothelial growth factor, hepatocyte growth factor, transforming growth factor, C-X-C motif chemokine ligand (CXCL)-1, and CXCL-2 in multilayered sheets was much higher than that in single-layered mixed-cell sheets (single-layered sheets) and multilayered sheets of fibroblasts alone (fibroblast sheets). The supernatant in multilayered sheets enhanced angiogenic potency and fibroblast migration compared with single-layered and fibroblast sheets in an in vitro experiment. The wound healing rate in the multilayered sheet-treated...Continue Reading

Citations

Jul 23, 2019·European Journal of Cardio-thoracic Surgery : Official Journal of the European Association for Cardio-thoracic Surgery·Akira FujitaKimikazu Hamano
Dec 7, 2018·Journal of the European Academy of Dermatology and Venereology : JEADV·C Chocarro-WronaJ A Marchal
Jul 29, 2020·International Journal of Molecular Sciences·Elena GroppaSerena Zacchigna
Sep 10, 2020·International Journal of Molecular Sciences·Jeannie DevereauxVasso Apostolopoulos
Mar 13, 2021·Seminars in Thoracic and Cardiovascular Surgery·Sota YoshimineKimikazu Hamano
May 19, 2021·Seminars in Thoracic and Cardiovascular Surgery·Agnes Y Choi, Chuong D Hoang
Jul 10, 2021·Advances in Wound Care·Walker D ShortSundeep G Keswani

❮ Previous
Next ❯

Related Concepts

Related Feeds

Arterial-Venous in Development & Disease

Arterial-venous development may play a crucial role in cardiovascular diseases. Here is the latest research.

Cell Migration

Cell migration is involved in a variety of physiological and pathological processes such as embryonic development, cancer metastasis, blood vessel formation and remoulding, tissue regeneration, immune surveillance and inflammation. Here is the latest research.