Treatment of fibroblast-like synoviocytes with IFN-gamma results in the down-regulation of autotaxin mRNA

Biochemical and Biophysical Research Communications
A N SantosJ Langner

Abstract

In an effort to isolate genes that change expression at the mRNA level during treatment of fibroblast-like synoviocytes (SFC) with IFN-gama, we performed a differential display analysis. Here, we report the isolation of a cDNA clone corresponding to a 3.1 kb mRNA species that is reduced in synoviocytes after culture with IFN-gama. Sequence analysis revealed the 211 bp length cDNA clone to be identical to the motility-stimulating 125 kDa protein autotaxin (ATX). The down-regulation of ATX mRNA was confirmed by Northern blot analysis as well as competitive RT-PCR. SFC express 1 ng ATX mRNA/microgram total RNA. IFN-gama down-regulated ATX mRNA up to 50% as compared to control. Our results add a new finding to the manifold functions described for IFN-gama in rheumatoid arthritis.

Citations

Nov 25, 2000·Clinical and Experimental Immunology·H TaubertD Riemann
Apr 12, 2012·The Journal of Experimental Medicine·Ioanna NikitopoulouVassilis Aidinis
Mar 15, 2013·Annals of the Rheumatic Diseases·Beatriz OrosaCarmen Conde
Sep 22, 2016·Osteoarthritis and Cartilage·J J McDougallM G Chambers
Feb 24, 2001·Critical Reviews in Biochemistry and Molecular Biology·M BollenC Stefan
Dec 17, 2009·Bioorganic & Medicinal Chemistry·Adrienne B HoeglundDaniel L Baker

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