Treatment of Helicobacter pylori with dielectric barrier discharge plasma causes UV induced damage to genomic DNA leading to cell death

Chemosphere
Akikazu SakudoTatsuya Misawa

Abstract

Gastrointestinal endoscopy is an important tool for the indentification and treatment of disorders of the gastrointestinal tract. However, nosocomial infections of Helicobacter pylori have been linked to the use of contaminated endoscopes. Disinfectants such as glutaraldehyde, ortho-phthalaldehyde and peracetic acid are generally used in the reprocesssing of endoscopes, but these chemicals are hazardous to human health. Thus, safer reprocessing and disinfecion methods are needed. In this study, we applied a dielectric barrier discharge (DBD) plasma torch for inactivation of H. pylori to investigate a potential new methodology to disinfect endoscopes. Suspensions of H. pylori in 10% glycerol were subjected to the DBD plasma torch, which reduced the viable cell count to undetectable levels after 2 min of treatment. Furthermore, urease activity of H. pylori was eliminated after 2 min-plasma treatment, while plasma-treatment reduced the intact DNA of H. pylori in a time-dependent manner. Next, we examined several potential bactericidal factors produced by the DBD plasma torch. Two min-plasma treatment resulted in a small temperature rise (4 °C), ultraviolet radiation (UV) generation, and the production of hydrogen peroxide. H. pylo...Continue Reading

Citations

May 16, 2019·Applied Microbiology and Biotechnology·Juliana ŠimončicováBarbora Kaliňáková
Jun 9, 2020·Frontiers in Microbiology·Yifan RaoXiancai Rao
Sep 4, 2020·International Journal of Molecular Sciences·Akikazu Sakudo, Tatsuya Misawa
Jun 3, 2021·International Journal of Molecular Sciences·Akikazu Sakudo, Yoshihito Yagyu
Aug 14, 2021·Chemosphere·Anelise Leal Vieira CubasElisa Helena Siegel Moecke

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