Abstract
The study aimed to evaluate whether mesenchymal stem cells transfected with bone morphogenetic protein (BMP) 2/7 could increase bone regeneration after radiotherapy using a rabbit model of mandibular distraction osteogenesis. Twelve rabbits were randomly assigned to the sham control, radiotherapy control, nontransfected mesenchymal stem cells (MSCs), and MSCs transfected with BMP-2/7 groups. All rabbits, except those in the sham control group, received preoperative radiation of 9 Gy for 5 fractions. One month after radiotherapy, all rabbits underwent unilateral mandibular distraction at a rate of 0.9 mm/d for 11 days. At the end of active distraction, MSCs combined with bovine collagen were injected into the distraction zone. After 4 weeks of consolidation, the mandibular samples were collected and subjected to radiographic, microcomputed tomographic, and histologic examinations. By radiographic examination, animals injected with nontransfected MSCs or MSCs encoding BMP-2/7 exhibited more bone formation than the control groups. Histologic examination showed that the group with MSCs encoding BMP-2/7 had a more mature medullary cavity than the nontransfected MSCs group. MSCs encoding BMP-2/7 can increase bone healing in irradiate...Continue Reading
References
Jan 1, 1994·Plastic and Reconstructive Surgery·A GantousD Holmberg
May 16, 2002·European Journal of Oral Sciences·Arja MuhonenRisto-Pekka Happonen
Oct 3, 2002·Archives of Oral Biology·Arja MuhonenRisto-Pekka Happonen
Oct 4, 2002·International Journal of Oral and Maxillofacial Surgery·A MuhonenR P Happonen
Sep 23, 2003·Bone·Reggie C HamdyThomas Steffen
Mar 11, 2004·Tissue Engineering·Kris A Partridge, Richard O C Oreffo
Mar 11, 2005·Journal of Cellular Biochemistry·Ming ZhaoRenny T Franceschi
Sep 29, 2005·Injury·Peter V Giannoudis, Christopher Tzioupis
Oct 29, 2005·Journal of Cranio-maxillo-facial Surgery : Official Publication of the European Association for Cranio-Maxillo-Facial Surgery·Angélique GirodPierre Bouletreau
Dec 13, 2005·The British Journal of Oral & Maxillofacial Surgery·Raúl González-GarcíaFrancisco J Díaz-González
Feb 4, 2006·The Lancet Oncology·James J Sciubba, David Goldenberg
Jun 3, 2006·The International Journal of Developmental Biology·Xuguang NiePaivi Kettunen
Jul 13, 2006·Annals of the New York Academy of Sciences·Xuelin Li, Xu Cao
Jul 31, 2007·Development, Growth & Differentiation·Benoît Robert
Sep 26, 2007·Expert Review of Anticancer Therapy·Daniel T T ChuaWilliam I Wei
Oct 4, 2007·The Journal of Craniofacial Surgery·Katsuhiko KashiwaYukihiko Arai
Oct 6, 2007·Journal of the American Veterinary Medical Association·Carl A Kirker-HeadKarl H Kraus
Dec 18, 2007·Molecular Cell·J Wade Harper, Stephen J Elledge
May 21, 2008·Archives of Facial Plastic Surgery·Daniel L PriceKeith M Furutani
Jun 20, 2008·The British Journal of Oral & Maxillofacial Surgery·Andrew Lyons, Naseem Ghazali
Aug 13, 2008·The Journal of Hand Surgery, European Volume·M R Thatte, R Mehta
Sep 25, 2008·The Journal of Craniofacial Surgery·Darren M SmithJoseph E Losee
Nov 27, 2008·Facial Plastic Surgery : FPS·Granger B WongBonnie L Padwa
Apr 21, 2009·Journal of Oral and Maxillofacial Surgery : Official Journal of the American Association of Oral and Maxillofacial Surgeons·Leonard B KabanMaria J Troulis
Mar 5, 2010·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Li MaLim Kwong Cheung
Citations
Apr 16, 2013·Tissue Engineering. Part B, Reviews·Asim M Makhdom, Reggie C Hamdy
Mar 14, 2014·The Journal of Craniofacial Surgery·Seung Yong SongYong Oock Kim
Apr 2, 2014·International Journal of Oral and Maxillofacial Surgery·K TamuraK Bessho
Apr 14, 2015·Orthodontics & Craniofacial Research·B C Tee, Z Sun
Jul 15, 2015·Cancer Letters·Nils H NicolayPeter E Huber
Jun 27, 2014·Nanomedicine : Nanotechnology, Biology, and Medicine·Asim M MakhdomReggie C Hamdy
Jul 11, 2018·World Journal of Stem Cells·Shiva SafariSaeed Reza Motamedian
Nov 22, 2018·Brazilian Oral Research·Carlos Manuel Rubio MorilloCristina Cunha Villar