Treatment of limb wounds of horses with orf virus IL-10 and VEGF-E accelerates resolution of exuberant granulation tissue, but does not prevent its development

PloS One
Lyn M WiseChristine L Theoret

Abstract

Bandaging of limb wounds in horses leads to formation of exuberant granulation tissue (EGT) that retards healing due to protracted inflammation, aberrant vascularisation and delayed epithelialisation. EGT is not observed if wounds are left undressed or when wounds are on the body. A previous study showed that short-term administration of proteins derived from orf virus dampened inflammation and promoted epithelialisation of open wounds in horses. Here, we investigated the impact of orf virus interleukin-10 and vascular endothelial growth factor-E on the development and resolution of EGT. Excisional wounds were created on the forelimb of four horses, and bandages were maintained until full healing to induce EGT formation. Matching body wounds were created to ensure EGT was limited to the limb, and to differentiate the effects of the viral proteins on normal healing and on EGT formation. Viral proteins or the hydrogel vehicle control were administered topically to site-matched wounds at day 1, with repeat administration at day 8. Wound healing and EGT formation were monitored macroscopically. Wound margin samples were harvested at 2, 7 and 14 days, and at full healing, with histology used to observe epithelialisation, immunofluor...Continue Reading

References

Apr 1, 1991·British Journal of Plastic Surgery·V Shakespeare, P Shakespeare
Feb 10, 1999·Equine Veterinary Journal·J M WilminkA Barneveld
Mar 17, 1999·Proceedings of the National Academy of Sciences of the United States of America·L M WiseS A Stacker
Mar 14, 2000·Vitamins and Hormones·H D BeerS Werner
May 15, 2001·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·T P SullivanP Mertz
Jun 1, 2002·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Dorothy M SuppSteven T Boyce
Jan 18, 2003·Journal of Trace Elements in Medicine and Biology : Organ of the Society for Minerals and Trace Elements (GMS)·Rosine Mayap NzietchuengKetsia Hess
Mar 12, 2003·Cell·Thorsten CramerRandall S Johnson
Aug 23, 2005·American Journal of Veterinary Research·Marilys Ducharme-DesjarlaisChristine L Theoret
Sep 24, 2005·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·Elodie LepaultChristine L Theoret
Mar 25, 2006·The Journal of Investigative Dermatology·Peter HelmboldRamesh C Nayak
Jun 24, 2006·Arteriosclerosis, Thrombosis, and Vascular Biology·Yujuan ZhengMasabumi Shibuya
Dec 30, 2006·Arteriosclerosis, Thrombosis, and Vascular Biology·Yujuan ZhengMasabumi Shibuya
Dec 7, 2007·Research in Veterinary Science·G BussièresE Troncy
Dec 19, 2007·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·Ashley GordonTimothy M Crombleholme
Jan 18, 2008·The Journal of Investigative Dermatology·William H PeranteauKenneth W Liechty
Sep 27, 2008·Current Protocols in Cell Biology·Monica A SerbanGlenn D Prestwich
Jun 6, 2009·Australian Veterinary Journal·A J DartP Canfield
Mar 6, 2010·The American Journal of Pathology·Cecelia C YatesAlan Wells
Oct 20, 2010·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·Christophe J CelesteChristine L Theoret
Mar 3, 2011·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·Karine DescheneChristine L Theoret
Feb 28, 2013·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·Christine L Theoret, Jacintha M Wilmink
May 1, 2013·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·Ingrid KieranMark Ferguson
Sep 11, 2013·Veterinary Surgery : VS·Christine L TheoretJohn Hicks
Mar 22, 2014·Frontiers in Immunology·Aaron C Petrey, Carol A de la Motte
Jan 1, 2013·Cells·Stuart J MillsPritinder Kaur
Apr 24, 2014·Advances in Wound Care·Alice KingSundeep G Keswani
May 23, 2014·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·Lyn M WiseAndrew A Mercer
Jun 18, 2014·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·Mette A SørensenStine Jacobsen
Oct 11, 2014·Advances in Wound Care·Kelly E Johnson, Traci A Wilgus
Sep 12, 2015·New Zealand Veterinary Journal·C L TheoretC B Riley
Nov 26, 2015·International Journal of Molecular Sciences·Kynan T Lawlor, Pritinder Kaur
Jul 6, 2016·The British Journal of Dermatology·G C LimandjajaS Gibbs

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Citations

Mar 2, 2019·Annual Review of Biomedical Engineering·Lucília P da SilvaAlexandra P Marques
Jul 9, 2020·Anatomia, histologia, embryologia·Juliane RiegerJohanna Plendl
Apr 16, 2020·Journal of Clinical Medicine·Lyn M WiseAndrew A Mercer
Apr 3, 2020·Journal of Avian Medicine and Surgery·Kelly RockwellThomas N Tully
May 16, 2019·Growth Factors·Zabeen Lateef, Lyn M Wise
Apr 5, 2020·Journal of Clinical Medicine·Jordan R YaronAlexandra R Lucas
May 28, 2021·Advances in Wound Care·Rebecca M HarmanGerlinde R Van de Walle

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Methods Mentioned

BETA
biopsy
biopsies
PCR
transgenic

Software Mentioned

Photoshop
SAS
ImageJ

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