Treatment of neutrophils with cytochalasins converts rolling to stationary adhesion on P-selectin

Journal of Cellular Physiology
S Sheikh, G B Nash

Abstract

We investigated how disruption of the actin cytoskeleton with cytochalasins modified adhesion of neutrophils rolling on a platelet monolayer in vitro at 37 degrees C. When perfused at a wall shear stress of 0.1 Pa over rolling cells, cytochalasin B, cytochalasin D and dihydro-cytochalasin B each induced dose-dependent (approximately 1-10 microg/ml) conversion to stationary attachment over minutes. Stopping was associated with cell elongation to a teardrop shape. Increased deformability of cytochalasin-treated cells was independently evidenced by more rapid entry into a micropipette. Spherical shape and rolling were reestablished concurrently on washout of the cytochalasins, while increasing the shear stress in the range 0.2 to 1.0 Pa induced tear-drop-shaped cells to restart rolling even in the continued presence of cytochalasin. When cells were pretreated with cytochalasin B, they attached efficiently at 0.1 Pa, rolled initially and only stopped after approximately 30 seconds when elongation had been established. Adhesion was selectin-mediated in the presence or absence of cytochalasin B, as judged by inhibition of attachment by antibody against P-selectin and failure of antibody against beta2-integrin CD18 to influence adhesi...Continue Reading

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Citations

Jul 29, 2008·American Journal of Physiology. Heart and Circulatory Physiology·Parag PawarKonstantinos Konstantopoulos
Aug 15, 2002·The Journal of Cell Biology·Tadayuki YagoRodger P McEver
Dec 13, 2002·American Journal of Physiology. Cell Physiology·Monica M BurdickKonstantinos Konstantopoulos
Jan 17, 2003·American Journal of Physiology. Cell Physiology·Owen J T McCartyKonstantinos Konstantopoulos
May 7, 2010·American Journal of Physiology. Cell Physiology·Tao YuDennis F Kucik
Jan 11, 2003·The Journal of Biological Chemistry·William HanleyKonstantinos Konstantopoulos
Mar 30, 2001·The Journal of Biological Chemistry·T E WilliamsC Zhu
Mar 16, 2017·Computer Methods in Biomechanics and Biomedical Engineering·Liqiang Lin, Xiaowei Zeng
May 26, 2004·Journal of Cell Science·William D HanleyKonstantinos Konstantopoulos
Jul 10, 2012·Biophysical Journal·Damir B Khismatullin, George A Truskey

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