Treatment of rapidly growing K-BALB and CT26 mouse tumours using Semliki Forest virus and its derived vector

Gene Therapy
J W P SmythG J Atkins

Abstract

To assess the potential of immune stimulation in combination with apoptosis induction by Semliki Forest virus (SFV) and its derived vector for tumour treatment, we have utilized the poorly immunogenic and rapidly growing K-BALB and CT26 murine tumour models. Both cell lines underwent apoptosis and expressed viral antigen when infected with the SFV4 strain of SFV, or recombinant SFV (rSFV) virus-like particles (VLPs) encoding the p62-6k viral structural proteins. VLPs were used to immunize groups of BALB/c and BALB/c nu/nu mice prior to subcutaneous tumour induction and treatment. Direct intratumoral injection of VLPs or SFV4 resulted in an immediate and intense inflammatory reaction in immunized groups that was not observed in naive groups until day 5 of treatment, and was not observed in nu/nu groups. A significantly higher level of tumour growth inhibition was observed in immunocompetent groups than in athymic mice. For K-BALB tumours, SFV4 treated groups showed greater inhibition than that observed in VLP-treated groups, with immunization prior to treatment enhancing the overall antitumour effect and immune response. No significant difference was observed in CT26 tumours between VLP and SFV4-treated groups, but prior immuniz...Continue Reading

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Citations

Nov 13, 2008·Expert Reviews in Molecular Medicine·Gregory J AtkinsBrian J Sheahan
Sep 30, 2008·Virology Journal·Andrew E FirthJohn F Atkins
Feb 7, 2007·Cancer Immunology, Immunotherapy : CII·Joanne LysaghtKingston H G Mills
Oct 2, 2014·Expert Review of Vaccines·Karl Ljungberg, Peter Liljeström
Sep 16, 2014·Expert Review of Vaccines·Kenneth Lundstrom
Jun 18, 2014·Viruses·Kenneth Lundstrom
Aug 10, 2010·Virus Research·Jose I QuetglasCristian Smerdou
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Jul 24, 2010·Neuropathology and Applied Neurobiology·F P RocheG J Atkins
Jul 31, 2013·Oncoimmunology·Erika VacchelliLorenzo Galluzzi
Aug 7, 2014·Oncoimmunology·Jonathan PolLorenzo Galluzzi
Jun 27, 2014·Frontiers in Oncology·Andrew NguyenYonghong Wan
Nov 10, 2016·Vaccines·Kenneth Lundstrom
Jun 21, 2005·Molecular Therapy : the Journal of the American Society of Gene Therapy·Juan R Rodriguez-MadozCristian Smerdou
Jun 1, 2009·Viruses·Kenneth Lundstrom
Apr 20, 2007·International Journal of Cancer. Journal International Du Cancer·Ann-Marie MäättäJarmo Wahlfors
Mar 11, 2006·The Journal of General Virology·Sareen E GalbraithGregory J Atkins

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