Treatment of Saos-2 osteosarcoma cells with diallyl trisulfide is associated with an increase in calreticulin expression

Experimental and Therapeutic Medicine
Wen-Peng XieChuan-Jie Li

Abstract

Diallyl trisulfide (DATS) is a natural organic sulfur compound that may be isolated from garlic and has strong anticancer activity. DATS has been demonstrated to upregulate the expression of calreticulin (CRT) in various types of human cancers, which is associated with the prognosis of cancer and its response to therapy. However, whether DATS has the same effect on human osteosarcoma cells is not known. Therefore, in the present study, Saos-2 human osteosarcoma cells were cultured with different concentrations of DATS (0, 25, 50 and 100 µmol/l) for 24 h, or with 50 µmol/l DATS for different time periods (0, 12, 24 and 36 h). Reverse transcription-quantitative polymerase chain reaction (RT-qPCR), western blotting and immunofluorescent staining were used to detect CRT mRNA and protein in the Saos-2 cells. Exposure to DATS changed the morphology and inhibited the growth of the Saos-2 cells, and its effects appeared to be concentration- and exposure time-dependent. The optimum concentration and exposure time of DATS were 50 µmol/l and 24 h, respectively. The levels of CRT mRNA and protein in the Saos-2 cells were significantly upregulated following exposure to DATS. The upregulation of CRT expression by DATS may be a mechanism unde...Continue Reading

References

Jan 1, 1997·Journal of Ethnopharmacology·N MartínM J Sepúlveda
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Feb 6, 2004·European Journal of Pharmacology·Suby OommenDevarajan Karunagaran
Dec 4, 2008·Annals of Surgical Oncology·Chiung-Nien ChenKing-Jen Chang
Jun 25, 2009·Anti-cancer Drugs·Yong Kui ZhangDong Mei Diao
Dec 24, 2010·Science Translational Medicine·Mark P ChaoIrving L Weissman
May 19, 2012·Acta Biochimica Et Biophysica Sinica·Wenjun LiFei Gao
Aug 7, 2014·Evidence-based Complementary and Alternative Medicine : ECAM·Ling XuChaoqin Yu
Jan 15, 2015·The Journal of Cell Biology
Aug 26, 2015·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Michael S IsakoffRichard Gorlick
Nov 29, 2015·The Orthopedic Clinics of North America·Megan E Anderson
May 1, 2016·The Journal of Biological Chemistry·Su-Hyeong KimShivendra V Singh
Aug 26, 2016·Cancer Science·Satoshi MatsukumaHiroaki Nagano
Nov 26, 2016·Women's Health·Shahla Masood
Dec 19, 2016·F1000Research·Stefan S BielackLeo Kager
Dec 25, 2016·Mutation Research. Genetic Toxicology and Environmental Mutagenesis·Ni ChenJun Wang
Jan 15, 2017·Journal of Orthopaedic Surgery and Research·Xiaojun HePeng Fu
Jan 21, 2017·Cancer Biomarkers : Section a of Disease Markers·Xu-Hua ZhangGuo-Qing Tan
May 5, 2017·Indian Journal of Medical and Paediatric Oncology : Official Journal of Indian Society of Medical & Paediatric Oncology·Shachi Jain TaranNagraj B Malipatil

❮ Previous
Next ❯

Citations

Nov 22, 2019·Anti-cancer Agents in Medicinal Chemistry·Rumana AhmadArvind K Srivastava

❮ Previous
Next ❯

Methods Mentioned

BETA
protein folding
PCR
protein assay

Software Mentioned

SPSS
Pro Plus
Image
ImageQuant TL

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.