Abstract
Tissue-specific stem cells from differentiating embryonic stem (ES) cells are both pluripotent and genetically flexible. Recent observations indicate that ES cells can differentiate into hepatocytes. Therefore, cell-based therapy can potentially be a therapeutic alternative to liver transplantation. In this study the treatment of acute liver failure in rats by transplantation of hepatocyte nuclear factor 4 (HNF4)-overexpressing ES cells was investigated. The HNF4 was transfected into ES cells and ES cell clones overexpressing HNF4 were selected. The levels of markers of hepatocyte differentiation, including albumin, transthyretin, glucose-6-phosphates (G-6-P) and SAPK/ERK kinase-1 (SEK1) mRNA, were tested in spontaneously differentiated HNF4-overexpressing ES cells by reverse transcription-polymerase chain reaction (RT-PCR). The ultrastructure of the spontaneously differentiated HNF4-overexpressing ES cells was examined by electron microscopy. To induce acute liver failure, Sprague-Dawley rats were subjected to 90% hepatectomy and given 5% oral dextrose. The rats were divided into three groups. The rats in the treatment group (n = 12) received intraliver injection of 2 x 10(7) undifferentiated HNF4-overexpressing ES cells from ...Continue Reading
References
Feb 9, 1992·Nature·C J KuoG R Crabtree
Dec 1, 1990·Genes & Development·F M SladekJ E Darnell
Jan 1, 1989·European Surgical Research. Europäische Chirurgische Forschung. Recherches Chirurgicales Européennes·J EmondD Houssin
Oct 1, 1987·Journal of Cellular Physiology·Y SudaS Aizawa
Oct 1, 1984·Molecular and Cellular Biology·J LeavittL Kedes
Jul 9, 1981·Nature·M J Evans, M H Kaufman
Nov 1, 1994·Mechanisms of Development·S TaravirasG Kelsey
Aug 2, 1994·Proceedings of the National Academy of Sciences of the United States of America·S A DuncanJ E Darnell
Apr 4, 1997·Science·G K Michalopoulos, M C DeFrances
Jun 15, 1997·Transplantation·J NakamuraI J Fox
Nov 6, 1998·Science·J A ThomsonJ M Jones
Nov 13, 1998·Proceedings of the National Academy of Sciences of the United States of America·M J ShamblottJ D Gearhart
May 15, 1999·Science·B E PetersenJ P Goff
Jan 5, 2000·Molecular and Cellular Endocrinology·B FunkensteinC L Brown
Feb 15, 2000·Journal of Hepatology·M IwaiK Kashima
Apr 5, 2000·Nature Biotechnology·B E ReubinoffA Bongso
Jun 20, 2000·Surgical Endoscopy·S K MittalC Lerner
Feb 17, 2001·Gastroenterology·E B Keeffe
Apr 28, 2001·Science·N LumelskyR McKay
May 30, 2001·FEBS Letters·T HamazakiN Terada
Dec 13, 2001·Experimental Cell Research·Elizabeth A JonesLesley M Forrester
Jun 27, 2002·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Ryoko ChinzeiHirobumi Teraoka
Apr 30, 2003·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Hanako YamamotoTakahiro Ochiya
Citations
Feb 11, 2009·Antioxidants & Redox Signaling·Dinender K Singla
Jan 27, 2007·Stem Cells and Development·Robert VincentJon Odorico
Jun 8, 2013·Journal of Cellular Biochemistry·Tao LiuYingjie Wang
May 29, 2016·Biochemical and Biophysical Research Communications·Neda YahooFardin Fathi
Sep 18, 2007·Expert Review of Neurotherapeutics·Dimitrios Karussis, Ibrahim Kassis
Jan 1, 2010·Cell Medicine·Luiz Anastácio AlvesRoberto Magalhães Saraiva