Treatment with antithymocyte globulin ameliorates intestinal ischemia and reperfusion injury in mice.

Surgery
M J WatsonD Farmer

Abstract

Antithymocyte therapy, specifically antithymocyte globulin (ATG; Thymoglobulin), is increasingly being used in organ transplantation to reduce allograft rejection. The T-lymphocyte has been purported to also play a role in ischemia and reperfusion injury (IRI); however, it has not been well studied. Our aim is to determine if ATG treatment impacts murine intestinal IRI. Under anesthesia, male C57BL6 mice underwent 100 minutes of warm intestinal IRI by clamping the superior mesenteric artery. The treatment group received rabbit anti-murine ATG (10 mg/kg) intraperitoneally 6 hours before IRI. Separate survival and analysis groups were performed. Intestinal tissue was procured at 4 and 24 hours after IRI. Tissue analysis included hematoxylin-eosin staining, CD3, CD4, and CD8 immunostaining, myeloperoxidase assay (MPO), quantitative real-time polymerase chain reaction studies, and Western blot. ATG treatment led to marked improvement in 7-day survival and a reduction in tissue injury by histology. MPO was also reduced, and immunostaining confirmed a significant reduction in CD3(+), CD4(+), and CD8(+) infiltrating cells in the treatment group. Quantitative real-time polymerase chain reaction analysis revealed the decreased expressio...Continue Reading

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Citations

Jun 5, 2013·The Journal of Surgical Research·Xuekang YangDahai Hu
Dec 18, 2013·International Reviews of Immunology·Dominik MeierGabriel E Gondolesi
May 18, 2016·The Journal of Immunology : Official Journal of the American Association of Immunologists·Michela FrascoliTippi C MacKenzie
Mar 2, 2013·Current Opinion in Organ Transplantation·Kaatje LenaertsSteven W M Olde Damink
May 20, 2015·The Journal of Immunology : Official Journal of the American Association of Immunologists·Wei-Feng LiuKe-Xuan Liu

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