Abstract
Antithymocyte therapy, specifically antithymocyte globulin (ATG; Thymoglobulin), is increasingly being used in organ transplantation to reduce allograft rejection. The T-lymphocyte has been purported to also play a role in ischemia and reperfusion injury (IRI); however, it has not been well studied. Our aim is to determine if ATG treatment impacts murine intestinal IRI. Under anesthesia, male C57BL6 mice underwent 100 minutes of warm intestinal IRI by clamping the superior mesenteric artery. The treatment group received rabbit anti-murine ATG (10 mg/kg) intraperitoneally 6 hours before IRI. Separate survival and analysis groups were performed. Intestinal tissue was procured at 4 and 24 hours after IRI. Tissue analysis included hematoxylin-eosin staining, CD3, CD4, and CD8 immunostaining, myeloperoxidase assay (MPO), quantitative real-time polymerase chain reaction studies, and Western blot. ATG treatment led to marked improvement in 7-day survival and a reduction in tissue injury by histology. MPO was also reduced, and immunostaining confirmed a significant reduction in CD3(+), CD4(+), and CD8(+) infiltrating cells in the treatment group. Quantitative real-time polymerase chain reaction analysis revealed the decreased expressio...Continue Reading
References
Nov 1, 1985·Journal of Pharmacological Methods·K M MullaneB Smith
Sep 29, 2000·Nephrology, Dialysis, Transplantation : Official Publication of the European Dialysis and Transplant Association - European Renal Association·D K YsebaertM E De Broe
Sep 23, 2003·Transplantation·William C GogginsA Benedict Cosimi
Oct 16, 2004·Digestive Diseases and Sciences·Ismail Hameed MallickAlexander M Seifalian
Feb 17, 2005·Transplantation·Douglas G FarmerJerzy Kupiec-Weglinski
May 13, 2005·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·Jorge ReyesThomas E Starzl
Jul 13, 2005·Clinical Transplantation·Diego BogettiEnrico Benedetti
Oct 8, 2005·Transplantation·Douglas G FarmerJerzy W Kupiec-Weglinski
Jun 3, 2006·Nephron. Experimental Nephrology·Vilmar Paiva MarquesNiels Olsen Saraiva Câmara
Nov 29, 2007·Nephrology, Dialysis, Transplantation : Official Publication of the European Dialysis and Transplant Association - European Renal Association·A MehrabiJ Schmidt
Jan 23, 2008·The Journal of Immunology : Official Journal of the American Association of Immunologists·Takashi HamadaAna J Coito
Oct 29, 2008·Transplant International : Official Journal of the European Society for Organ Transplantation·Kareem M Abu-ElmagdGeorge Mazariegos
Dec 2, 2008·The Journal of Surgical Research·Niteen TapuriaBrian R Davidson
Dec 2, 2008·Transplant Immunology·A Beiras-FernandezE Thein
Dec 23, 2008·Transplantation Proceedings·M J WatsonD G Farmer
May 23, 2009·Transplantation·Masaru KawaiJacques Pirenne
Sep 4, 2009·The New England Journal of Medicine·Thomas M Fishbein
Apr 17, 2010·Drugs·A Osama GaberMohamad Mohty
Apr 28, 2010·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·G V MazariegosJ C Magee
Nov 26, 2010·Transplantation·Douglas G FarmerRonald W Busuttil
Citations
Jun 5, 2013·The Journal of Surgical Research·Xuekang YangDahai Hu
Feb 18, 2016·Stem Cells International·Željka Večerić-HalerMartina Perše
Dec 18, 2013·International Reviews of Immunology·Dominik MeierGabriel E Gondolesi
Jul 14, 2017·PloS One·Yi YuChristian Klemann
Dec 3, 2016·The Journal of Biological Chemistry·Gospel Enyindah-AsonyeFeng Lin
May 18, 2016·The Journal of Immunology : Official Journal of the American Association of Immunologists·Michela FrascoliTippi C MacKenzie
Mar 2, 2013·Current Opinion in Organ Transplantation·Kaatje LenaertsSteven W M Olde Damink
May 20, 2015·The Journal of Immunology : Official Journal of the American Association of Immunologists·Wei-Feng LiuKe-Xuan Liu
Sep 11, 2018·Frontiers in Immunology·Paul V RitschlKatja Kotsch
Jul 3, 2021·Journal of Clinical Medicine·Paul Viktor RitschlJohann Pratschke
Sep 22, 2021·Scientific Reports·Dominik FunkenChristian Klemann