Treatment with EUK-134 improves sarcoplasmic reticulum Ca2+ release but not myofibrillar Ca2+ sensitivity after fatiguing contraction of rat fast-twitch muscle

American Journal of Physiology. Regulatory, Integrative and Comparative Physiology
Daiki WatanabeMasanobu Wada

Abstract

Skeletal muscles undergoing vigorous activity can enter a state of prolonged low-frequency force depression (PLFFD). This study was conducted to examine whether antioxidant treatment is capable of accelerating the recovery from PLFFD, with a focus on the function of the sarcoplasmic reticulum (SR) and myofibril. One hour before fatiguing stimulation (FS) was administered, rats received an intraperitoneal injection of Eukarion (EUK-134), which mimics the activities of superoxide dismutase and catalase. Intact muscles of the hindlimbs were electrically stimulated via the sciatic nerve until the force was reduced to ~50% of the initial force (FS). Thirty minutes after cessation of FS, the superficial regions of gastrocnemius muscles were dissected and used for biochemical and skinned-fiber analyses. Whole muscle analyses revealed that antioxidant alleviated the FS-induced decrease in the reduced glutathione content. Skinned-fiber analyses showed that the antioxidant did not affect the FS-induced decrease in the ratio of force at 1 Hz to that at 50 Hz. However, the antioxidant partially inhibited the FS-mediated decrease in the ratio of depolarization-induced force to the maximum Ca2+-activated force. Furthermore, the antioxidant c...Continue Reading

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Nov 3, 2016·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Daiki Watanabe, Masanobu Wada

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Citations

Jan 10, 2020·Journal of Applied Physiology·Daiki WatanabeYutaka Kano
Mar 19, 2020·Scandinavian Journal of Medicine & Science in Sports·Arthur J ChengTomas Venckunas
Jun 20, 2020·Journal of Applied Physiology·Hiroaki EshimaKatsuhiko Funai
Mar 21, 2019·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Thomas Chaillou, Arthur J Cheng
Sep 22, 2020·Journal of Cachexia, Sarcopenia and Muscle·Arthur J ChengHåkan Westerblad
Feb 18, 2021·American Journal of Physiology. Cell Physiology·Daiki Watanabe, Masanobu Wada
Aug 28, 2021·International Journal of Molecular Sciences·Nicholas A ForemanLi Li Ji
Mar 18, 2020·Redox Biology·Arthur J ChengJohanna T Lanner
Oct 21, 2021·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Jiayu ShiMasanobu Wada

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