Treatment with oleic acid reduces IgE binding to peanut and cashew allergens

Food Chemistry
Si-Yin ChungWendy A Desormeaux

Abstract

Oleic acid (OA) is known to bind and change the bioactivities of proteins, such as α-lactalbumin and β-lactoglobulin in vitro. The objective of this study was to determine if OA binds to allergens from a peanut extract or cashew allergen and changes their allergenic properties. Peanut extract or cashew allergen (Ana o 2) was treated with or without 5mM sodium oleate at 70°C for 60 min (T1) or under the same conditions with an additional overnight incubation at 37°C (T2). After treatment, the samples were dialyzed and analyzed by SDS-PAGE and for OA content. IgE binding was evaluated by ELISA and western blot, using a pooled serum or plasma from individuals with peanut or cashew allergies. Results showed that OA at a concentration of 5mM reduced IgE binding to the allergens. Peanut sample T2 exhibited a lower IgE binding and a higher OA content (protein-bound) than T1. Cashew allergen T2 also showed a reduction in IgE binding. We conclude that OA reduces the allergenic properties of peanut extract and cashew allergen by binding to the allergens. Our findings indicate that OA in the form of sodium oleate may be potentially useful as a coating to reduce the allergenic properties of peanut and cashew allergens.

References

Aug 15, 1995·Proceedings of the National Academy of Sciences of the United States of America·A HåkanssonC Svanborg
Feb 7, 2002·Journal of Agricultural and Food Chemistry·Si-Yin ChungDaniel W Gorbet
Aug 7, 2004·Clinical Nutrition : Official Journal of the European Society of Parenteral and Enteral Nutrition·Maria Fernanda Cury-BoaventuraRui Curi
Jan 4, 2005·Neurochemistry International·Yuan ZhuJosef Krieglstein
Nov 30, 2007·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·I-Chun LinCheng-Deng Kuo
Apr 30, 2011·The Journal of Biological Chemistry·Giorgia De FranceschiPatrizia Polverino de Laureto
Aug 9, 2011·Journal of the American Academy of Dermatology·Zain Husain, Robert A Schwartz
Aug 29, 2012·Journal of Agricultural and Food Chemistry·Solène Le MauxAndré Brodkorb
Feb 19, 2013·International Journal of Dermatology·Zain Husain, Robert A Schwartz
Feb 28, 2013·The FEBS Journal·Christel R BrinkmannDaniel E Otzen
Mar 19, 2013·Biochimica Et Biophysica Acta·Angelo FontanaPatrizia Polverino de Laureto
May 17, 2013·Allergy and Asthma Proceedings·Matthew J Greenhawt
May 25, 2013·Current Pharmaceutical Design·Si-Yin Chung, Shawndrika Reed
Feb 26, 2014·The Journal of Allergy and Clinical Immunology. in Practice·Richard L WassermanArnon Elizur
Feb 26, 2014·The Journal of Allergy and Clinical Immunology. in Practice·Robert A Wood, Hugh A Sampson
Apr 16, 2014·Food Chemistry·Adrián A PerezLiliana G Santiago
Jun 14, 2014·Journal of Agricultural and Food Chemistry·Christopher P MattisonCasey C Grimm
Jul 24, 2014·Food Chemistry·Si-Yin Chung, Shawndrika Reed
Jul 24, 2014·Food Chemistry·Claudia BelingheriElena Vittadini

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Citations

Sep 3, 2016·Clinical Reviews in Allergy & Immunology·Cíntia MendesIsabel Mafra
Apr 30, 2019·Critical Reviews in Food Science and Nutrition·Rosina López-Fandiño
Dec 1, 2017·Food Science & Nutrition·Si-Yin ChungShawndrika Reed

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