Treatment with Phytoestrogens Reversed Triclosan and Bisphenol A-Induced Anti-Apoptosis in Breast Cancer Cells

Biomolecules & Therapeutics
Geum-A LeeKyung-A Hwang

Abstract

Triclosan (TCS) and bisphenol A (BPA) are endocrine-disrupting chemicals that interfere with the hormone or endocrine system and may cause cancer. Kaempferol (Kaem) and 3,3'-diindolylmethane (DIM) are phytoestrogens that play chemopreventive roles in the inhibition of carcinogenesis and cancer progression. In this study, the influence of TCS, BPA, Kaem, and DIM on proliferation and apoptotic abilities of VM7Luc4E2 breast cancer cells were examined. MTT assay revealed that TCS (0.1-10 µM), BPA (0.1-10 µM) and E2 (0.01-0.0001 µM) induced significant cell proliferation of VM7Luc4E2 cells, which was restored to the control (0.1% DMSO) by co-treatment with Kaem (30 µM) or DIM (15 µM). Reactive oxygen species (ROS) production assays showed that TCS and BPA inhibited ROS production of VM7Luc4E2 cells similar to E2, but that co-treatment with Kaem or DIM on VM7Luc4E2 cells induced increased ROS production. Based on these results, the effects of TCS, BPA, Kaem, and DIM on protein expression of apoptosis and ROS production-related markers such as Bax and Bcl-xl, as well as endoplasmic reticulum (ER) stress-related markers such as eIF2α and CHOP were investigated by Western blot assay. The results revealed that TCS, and BPA induced anti-a...Continue Reading

References

Aug 13, 1999·Genes & Development·A GrossS J Korsmeyer
Sep 1, 1999·British Journal of Cancer·L Hilakivi-ClarkeR Clarke
Apr 27, 2001·Nature Structural Biology·Y Shi
Nov 17, 2001·Science·S W Fesik, Y Shi
Feb 19, 2002·Environmental Toxicology·Min-Yu ChenMasanori Fujita
Apr 6, 2004·Maturitas·Herjan J T Coelingh Bennink
Dec 17, 2004·Journal of the National Cancer Institute·Stacey A MissmerSusan E Hankinson
Mar 9, 2006·Best Practice & Research. Clinical Endocrinology & Metabolism·P D Darbre
Jul 20, 2007·Endocrine-related Cancer·Richard J SantenUNKNOWN Breast Cancer Prevention Collaborative Group
Jun 13, 2009·Breast Cancer Research : BCR·Joan S Lewis-Wambi, V Craig Jordan
Feb 5, 2010·Biometals : an International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine·Masanori Kitamura, Nobuhiko Hiramatsu
Dec 17, 2010·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Zohreh GalehdarSean P Cregan
Nov 15, 2011·Cell·Yaron Fuchs, Hermann Steller
Mar 22, 2012·BMC Research Notes·Simplice Joel Ndendoung TatsimoPierre Tane
Jun 27, 2012·The Journal of Cell Biology·Shiyu Wang, Randal J Kaufman
Jul 3, 2013·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Nam-Hee KangKyung-Chul Choi
Nov 23, 2013·Biomolecules & Therapeutics·Yeo-Jung KwonYoung-Jin Chun
Feb 26, 2014·International Journal of Environmental Research and Public Health·Michael T DinwiddieJiangang Chen
Aug 19, 2014·Bioscience, Biotechnology, and Biochemistry·Hee-Seok LeeJin-Hwan Hong
May 23, 2015·Nutrition and Cancer·Kyung-A Hwang, Kyung-Chul Choi
Aug 19, 2015·Frontiers in Oncology·Yoon-Hee HongYong Sang Song
Nov 26, 2015·Food Chemistry·Maria RussoSeyed Mohammad Nabavi
Oct 12, 2016·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Boyu YangXiaoling Liang
Dec 8, 2016·International Journal of Experimental Pathology·Sahar El-Sayed ElswefyRehab Abdallah Hasan

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Citations

Aug 6, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Danielly C Ferraz da CostaEliane Fialho
Sep 22, 2020·Journal of Environmental Science and Health. Part C, Toxicology and Carcinogenesis·Prabin ShresthaTit-Yee Wong
May 21, 2020·International Journal of Molecular Sciences·Magdalena ZaborowskaAgata Borowik
Jun 14, 2019·Oxidative Medicine and Cellular Longevity·Mohammad A Alfhili, Myon-Hee Lee
Jun 30, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Muhammad ImranLeticia M Estevinho
Jan 3, 2021·Apoptosis : an International Journal on Programmed Cell Death·Mohammad A AlfhiliShaw M Akula
Dec 18, 2020·Frontiers in Nutrition·Barbara J StillwaterOrnella I Selmin
Aug 28, 2021·International Journal of Molecular Sciences·Xiaoqiang WangShiuan Chen

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Methods Mentioned

BETA
protein folding
ELISA
fluorescence microscopy

Software Mentioned

Graph
Cell Sens Dimension
pad Prism

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