Treatment with the non-ionic surfactant poloxamer P188 reduces DNA fragmentation in cells from bovine chondral explants exposed to injurious unconfined compression

Biomechanics and Modeling in Mechanobiology
D C BaarsR C Haut

Abstract

Excessive mechanical loading to a joint has been linked with the development of post-traumatic osteoarthritis (OA). Among the suspected links between impact trauma to a joint and associated degeneration of articular cartilage is an acute reduction in chondrocyte viability. Recently, the non-ionic surfactant poloxamer 188 (P188) has been shown to reduce by approximately 50% the percentage of non-viable chondrocytes 24 h post-injury in chondral explants exposed to 25 MPa of unconfined compression. There is a question whether these acutely 'saved' chondrocytes will continue to degrade over time, as P188 is only thought to act by acute repair of damaged cell membranes. In order to investigate the degradation of traumatized chondrocytes in the longer term, the current study utilized TUNEL staining to document the percentage of cells suffering DNA fragmentation with and without an immediate 24 h period of exposure of the explants to P188 surfactant. In the current study, as in the previous study by this laboratory, chondral explants were excised from bovine metacarpophalangeal joints and subjected to 25 MPa of unconfined compression. TUNEL staining was performed at 1 h, 4 days, and 7 days post-impact. The current study found that P18...Continue Reading

References

May 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·R C LeeR L Wollmann
May 31, 1994·Annals of the New York Academy of Sciences·J T PadanilamM Toner
May 1, 1995·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·B Grasl-KrauppR Schulte-Hermann
Nov 1, 1995·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·P J Atkinson, R C Haut
Dec 1, 1996·Scientific American·R C DukeJ D Young
Apr 9, 2001·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·J D MarksR C Lee
May 12, 2001·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·T M QuinnE B Hunziker
Jul 31, 2001·Journal of Biomechanical Engineering·P J Atkinson, R C Haut
Aug 24, 2001·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·C T ChenG Lust
Sep 20, 2001·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·B J EwersR C Haut
Feb 14, 2002·Osteoarthritis and Cartilage·D D D'LimaM K Lotz
Feb 16, 2002·Cellular and Molecular Life Sciences : CMLS·A KerinA Grodzinsky
Sep 5, 2002·The Journal of Pathology·Shawn Patrick GroganPierre Mainil-Varlet
Aug 16, 2003·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Jack L LewisTheodore R Oegema
Dec 6, 2003·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Véronique Morel, Thomas M Quinn
Mar 31, 2004·Radiologic Clinics of North America·David T Felson
Apr 22, 2004·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·John G CostourosHubert T Kim
Aug 12, 2004·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Daniel M Phillips, Roger C Haut

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Citations

Nov 4, 2006·Biomechanics and Modeling in Mechanobiology·Gerard A Ateshian
Oct 23, 2010·Biomechanics and Modeling in Mechanobiology·Ayşegül KavasAyşen Tezcaner
Aug 5, 2009·The Journal of Bone and Joint Surgery. American Volume·James A MartinTodd O McKinley
May 29, 2008·Haemophilia : the Official Journal of the World Federation of Hemophilia·N W D JansenF P J G Lafeber
Jun 5, 2008·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Feng WeiRoger C Haut
Sep 20, 2011·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Ellen SauterJames A Martin
Oct 31, 2009·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Daniel I IsaacRoger C Haut
Oct 17, 2015·Arthritis & Rheumatology·Mitchell C ColemanJames A Martin
Apr 8, 2015·Journal of the Mechanical Behavior of Biomedical Materials·Garrett A CoatneyTammy L Haut Donahue
May 8, 2013·Journal of Controlled Release : Official Journal of the Controlled Release Society·Marie MorilleClaudia N Montero-Menei
Oct 30, 2009·The American Journal of Sports Medicine·Michal SzczodryConstance R Chu
Nov 27, 2009·The American Journal of Sports Medicine·Bruce Reider
May 29, 2008·Clinical Orthopaedics and Related Research·Hubert T KimAlexis C Dang
Jul 8, 2008·Journal of Biomechanical Engineering·Roman M Natoli, Kyriacos A Athanasiou
Jan 8, 2011·Physical Biology·Leida G AbraçadoMarcos Farina
Feb 24, 2021·Journal of the Mechanical Behavior of Biomedical Materials·Gerardo E NarezTammy L Haut Donahue

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