Abstract
Trefoil factor 3 (TFF3) is a secreted protein which functions in mucosal repair of the gastrointestinal tract. This is achieved through the combined stimulation of cell migration and prevention of apoptosis and anoikis, thus facilitating repair. Deregulated TFF3 expression at the gene and protein level is implicated in numerous cancers. In prostate cancer TFF3 has previously been reported as a potential biomarker, overexpressed in a subset of primary and metastatic cases. Here we investigated the effect of increased TFF3 expression on prostate cancer cell behaviour. Oncomine analysis demonstrated that TFF3 mRNA expression was upregulated in prostate cancer compared to normal tissue. Forced-expression models were established in the prostate cancer cell lines, DU145 and PC3, by stable transfection of an expression vector containing the TFF3 cDNA. Forced expression of TFF3 significantly increased total cell number and cell viability, cell proliferation and cell survival. In addition, TFF3 enhanced anchorage independent growth, 3-dimensional colony formation, wound healing and cell migration compared to control transfected cell lines. We also observed reduced sensitivity to ionising radiation in stably transfected cell lines. In do...Continue Reading
References
Sep 24, 1999·Reproduction, Nutrition, Development·A Ferlay, Y Chilliard
Sep 25, 2003·Nature Reviews. Molecular Cell Biology·Douglas Taupin, Daniel K Podolsky
Apr 6, 2004·The Prostate·Alexis ScherlNicole Schreiber-Agus
Jun 5, 2004·Peptides·Dirk Meyer zum BüschenfeldeOtmar Huber
Jun 5, 2004·Peptides·Shahin EmamiChristian Gespach
Sep 16, 2004·The Prostate·Isla P GarrawayRobert E Reiter
Sep 16, 2004·The Prostate·Dennis A FaithAngelo M De Marzo
Dec 24, 2005·Cellular and Molecular Life Sciences : CMLS·G RegaloJ C Machado
Dec 24, 2005·Cellular and Molecular Life Sciences : CMLS·M Baus-Loncar, A S Giraud
Dec 24, 2005·Cellular and Molecular Life Sciences : CMLS·W Hoffmann
Dec 24, 2005·Cellular and Molecular Life Sciences : CMLS·L Thim, F E B May
Feb 10, 2006·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Else Marie VestergaardNiels Tørring
Jan 24, 2007·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Jens MadsenUffe Holmskov
Dec 7, 2007·Trends in Endocrinology and Metabolism : TEM·Jo K PerryPeter E Lobie
Jul 11, 2009·Endocrinology·Naeem AmiryPeter E Lobie
Jan 30, 2010·International Journal of Cancer. Journal International Du Cancer·Else Marie VestergaardKarina Dalsgaard Sørensen
Aug 6, 2010·The Prostate·F Gallardo-ArrietaJ Reventós
Jul 12, 2011·International Journal of Molecular Medicine·Zaki ShaikhibrahimNicolas Wernert
Jul 25, 2014·Therapeutic Advances in Medical Oncology·Daniel L Suzman, Emmanuel S Antonarakis
Citations
Sep 15, 2016·PloS One·Jan Große-KreulNicole Dünker
Nov 12, 2016·Cancer Gene Therapy·Z YuanY Wei
Sep 21, 2017·International Journal of Molecular Sciences·Maibritt NørgaardKarina Dalsgaard Sørensen
Oct 20, 2015·Biomolecular Concepts·Maike Busch, Nicole Dünker
Apr 27, 2017·Oncotarget·Ming-Liang YouPeter E Lobie
Nov 2, 2017·Oncotarget·Qing-Yun ChongPeter E Lobie
Nov 5, 2017·Oncotarget·Vijay PandeyPeter E Lobie
Jul 12, 2020·Journal of Cellular and Molecular Medicine·Huaiguo WuXuedong Wang
Nov 20, 2016·Clinical and Experimental Pharmacology & Physiology·Yongchang GuanZhi Cao
Apr 30, 2017·Cancer Biomarkers : Section a of Disease Markers·Boram ChoiHan-Kwang Yang
Oct 17, 2018·Nature Communications·Richard Peter OwenXin Lu
Aug 25, 2018·Experimental & Molecular Medicine·Jieying LiuYeun-Jun Chung
Oct 30, 2019·Cancers·Han Ming PohPeter E Lobie
Dec 15, 2019·International Journal of Molecular Sciences·Ru-Mei ChenPeter E Lobie
Nov 7, 2019·Oncogenesis·Mengyi ZhangPeter E Lobie
Aug 23, 2019·International Journal of Oncology·Aikeremu YusufuHaijiang Wang
Jan 11, 2017·Cancer Cell International·Zhaohu YuanYaming Wei
Dec 1, 2017·Cancer Research·Natalia Martín-MartínArkaitz Carracedo
Dec 11, 2020·BMC Cancer·Pushpaja DodlaSuganthi Sridhar
Jan 29, 2021·World Journal of Gastroenterology : WJG·Yun HuangShi-Nan Nie