Trefoil factor 3 (TFF3) enhances the oncogenic characteristics of prostate carcinoma cells and reduces sensitivity to ionising radiation

Cancer Letters
Omesha PereraJo K Perry

Abstract

Trefoil factor 3 (TFF3) is a secreted protein which functions in mucosal repair of the gastrointestinal tract. This is achieved through the combined stimulation of cell migration and prevention of apoptosis and anoikis, thus facilitating repair. Deregulated TFF3 expression at the gene and protein level is implicated in numerous cancers. In prostate cancer TFF3 has previously been reported as a potential biomarker, overexpressed in a subset of primary and metastatic cases. Here we investigated the effect of increased TFF3 expression on prostate cancer cell behaviour. Oncomine analysis demonstrated that TFF3 mRNA expression was upregulated in prostate cancer compared to normal tissue. Forced-expression models were established in the prostate cancer cell lines, DU145 and PC3, by stable transfection of an expression vector containing the TFF3 cDNA. Forced expression of TFF3 significantly increased total cell number and cell viability, cell proliferation and cell survival. In addition, TFF3 enhanced anchorage independent growth, 3-dimensional colony formation, wound healing and cell migration compared to control transfected cell lines. We also observed reduced sensitivity to ionising radiation in stably transfected cell lines. In do...Continue Reading

References

Sep 25, 2003·Nature Reviews. Molecular Cell Biology·Douglas Taupin, Daniel K Podolsky
Jun 5, 2004·Peptides·Shahin EmamiChristian Gespach
Sep 16, 2004·The Prostate·Isla P GarrawayRobert E Reiter
Dec 24, 2005·Cellular and Molecular Life Sciences : CMLS·G RegaloJ C Machado
Dec 24, 2005·Cellular and Molecular Life Sciences : CMLS·M Baus-Loncar, A S Giraud
Dec 24, 2005·Cellular and Molecular Life Sciences : CMLS·W Hoffmann
Dec 24, 2005·Cellular and Molecular Life Sciences : CMLS·L Thim, F E B May
Feb 10, 2006·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Else Marie VestergaardNiels Tørring
Jan 24, 2007·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Jens MadsenUffe Holmskov
Dec 7, 2007·Trends in Endocrinology and Metabolism : TEM·Jo K PerryPeter E Lobie
Jul 11, 2009·Endocrinology·Naeem AmiryPeter E Lobie
Jan 30, 2010·International Journal of Cancer. Journal International Du Cancer·Else Marie VestergaardKarina Dalsgaard Sørensen
Jul 12, 2011·International Journal of Molecular Medicine·Zaki ShaikhibrahimNicolas Wernert
Jul 25, 2014·Therapeutic Advances in Medical Oncology·Daniel L Suzman, Emmanuel S Antonarakis

❮ Previous
Next ❯

Citations

Sep 21, 2017·International Journal of Molecular Sciences·Maibritt NørgaardKarina Dalsgaard Sørensen
Oct 20, 2015·Biomolecular Concepts·Maike Busch, Nicole Dünker
Nov 20, 2016·Clinical and Experimental Pharmacology & Physiology·Yongchang GuanZhi Cao
Apr 30, 2017·Cancer Biomarkers : Section a of Disease Markers·Boram ChoiHan-Kwang Yang
Aug 25, 2018·Experimental & Molecular Medicine·Jieying LiuYeun-Jun Chung
Dec 1, 2017·Cancer Research·Natalia Martín-MartínArkaitz Carracedo
Jan 29, 2021·World Journal of Gastroenterology : WJG·Yun HuangShi-Nan Nie

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cadherins and Catenins

Cadherins (named for "calcium-dependent adhesion") are a type of cell adhesion molecule (CAM) that is important in the formation of adherens junctions to bind cells with each other. Catenins are a family of proteins found in complexes with cadherin cell adhesion molecules of animal cells: alpha-catenin can bind to β-catenin and can also bind actin. β-catenin binds the cytoplasmic domain of some cadherins. Discover the latest research on cadherins and catenins here.

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis

Apoptosis in Cancer

Apoptosis is an important mechanism in cancer. By evading apoptosis, tumors can continue to grow without regulation and metastasize systemically. Many therapies are evaluating the use of pro-apoptotic activation to eliminate cancer growth. Here is the latest research on apoptosis in cancer.

Cell Migration

Cell migration is involved in a variety of physiological and pathological processes such as embryonic development, cancer metastasis, blood vessel formation and remoulding, tissue regeneration, immune surveillance and inflammation. Here is the latest research.

Cell Migration in Cancer and Metastasis

Migration of cancer cells into surrounding tissue and the vasculature is an initial step in tumor metastasis. Discover the latest research on cell migration in cancer and metastasis here.