Trehalose in glycerol-free freezing extender enhances post-thaw survival of boar spermatozoa

The Journal of Reproduction and Development
Rukmali AthurupanaHiroaki Funahashi

Abstract

Cryopreservation of boar semen is still considered suboptimal due to lower fertility as compared with fresh samples when glycerol, a permeating cryoprotectant, is used. Trehalose is a non-permeable cryoprotectant and nonreducing disaccharide known to stabilize proteins and biologic membranes. The aim of this study was to evaluate the cryosurvival and in vitro penetrability of boar spermatozoa when glycerol was replaced with trehalose in a freezing extender. Ejaculated Berkshire semen samples were diluted in egg yolk-based freezing extender containing glycerol (100 mM) or trehalose (0, 50, 100, 150, 200 and 250 mM) and cryopreserved using a straw freezing procedure. Thawed samples were analyzed for motility, viability, mitochondrial membrane potential (MMP), and acrosome integrity. In experiment 2, penetrability of spermatozoa cryopreserved with 100 mM glycerol or trehalose was examined. Replacement of cryoprotectant glycerol (100 mM) with trehalose had no effect on sperm viability, but replacing it with 100 mM trehalose improved motility, MMP and acrosome integrity significantly. Sperm motility and MMP were considerably higher in 100 mM trehalose, whereas the acrosome integrity was substantially higher in 100-250 mM trehalose. ...Continue Reading

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Citations

Jul 16, 2015·Reproduction in Domestic Animals = Zuchthygiene·H Funahashi
Aug 21, 2020·Biopreservation and Biobanking·Nazan KeskinSukru Dursun
May 13, 2021·The Journal of Physical Chemistry. B·Daniel J KozuchPablo G Debenedetti
May 28, 2021·Biopreservation and Biobanking·Elaheh ShahmoradiIman Halvaei

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