Trek1 contributes to maintaining nasal epithelial barrier integrity

Scientific Reports
Jing JiangPing-Chang Yang

Abstract

Epithelial barrier integrity is critical to maintain the homeostasis in the body. The regulatory mechanism of the epithelial barrier function has not been fully understood. This study aims to elucidate the role of the TWIK-related potassium channel-1 (Trek1) in the regulation of the epithelial barrier function of the nasal mucosa. In this study, the levels of Trek1 were assessed by real time RT-PCR and Western blotting. The epithelial barrier function of the rat nasal epithelia was evaluated by the Ussing chamber system. The results showed that Trek1 was detected in the human and rat nasal epithelia, which were significantly lower in patients and rats with allergic rhinitis than that in healthy controls. Exposure to the signature T helper 2 cytokine, interleukin (IL)-4, markedly suppressed the expression of Trek1 in the nasal mucosa via up regulating the expression of the histone deacetylase (HDAC)1. The IL-4-induced rat nasal epithelial barrier dysfunction could be blocked by HDAC1 inhibitor (Trichostatin A), or sodium butyrate, or administration of Clostridium Butyricum. We conclude that Trek1 is critical to maintain the nasal epithelial barrier function.

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Citations

Feb 21, 2016·Pharmacology & Therapeutics·Susumu OhyaKatsuhiko Muraki
May 13, 2017·Immunity, Inflammation and Disease·Caroline SandenJonas S Erjefält
Sep 22, 2020·PeerJ·Che Othman Siti SarahKah Keng Wong

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electrophoresis

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