Trend analysis of the role of circular RNA in goat skeletal muscle development

BMC Genomics
Yinghui LingXiaorong Zhang

Abstract

Circular RNA (circRNA) is produced during the splicing of mRNA (in addition to linear splicing) and is part of the gene regulatory network. The temporal expression patterns the different developmental stages were inseparable from these molecules' function. Skeletal muscles of Anhui white goat (AWG) across seven fetal to postnatal development stages were sequenced and 21 RNA sequencing libraries were constructed. We thereby identified 9090 circRNAs and analyzed their molecular properties, temporal expression patterns, and potential functions at the different stages. CircRNAs showed complexities and diversity of formation as the same host gene produces multiple isoforms of these nucleic acids with different expression profiles. The differential expression of 2881 circRNAs (DECs, P < 0.05) was identified and four were randomly selected and validated by qPCR. Moreover, 1118 DECs under strict selected (SDECs, |log2FC| > 2 and P-adj value < 0.01) showed 4 expression trends (Clusters 0, 19, 16 and 18). Cluster 0 molecules had increasing expression at all stages with effects on muscle through metabolism, regulation of enzyme activity, and biosynthesis. Cluster 16 circRNAs had high expression in the early and late stages and are involve...Continue Reading

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Citations

Sep 12, 2020·Reproduction in Domestic Animals = Zuchthygiene·Yong HuangXihong Sun
Mar 23, 2021·Frontiers in Cell and Developmental Biology·Zhenguo YangQingyun Chen
Jul 21, 2021·International Journal of Molecular Sciences·Golam RbbaniJorge M O Fernandes

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Methods Mentioned

BETA
RNA-seq
cesarean section
RNA Assay

Software Mentioned

Stem
BLAST
Bowtie
house Perl scripts
circ
SPSS
find
Primer
Image Pro®
GOseq R package

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