TRESK background potassium channel is not gated at the helix bundle crossing near the cytoplasmic end of the pore

PloS One
Miklós LengyelPéter Enyedi

Abstract

Two-pore domain K+ channels (K2P) are responsible for background K+ currents and regulate the resting membrane potential and cellular excitability. Their activity is controlled by a large variety of physicochemical factors and intracellular signaling pathways. The majority of these effects converge on the intracellular C-terminus of the channels, resulting in the modification of the gating at the selectivity filter. Another gating mechanism, the activation gate at the helix bundle crossing is also well documented in other K+ channel families, however, it remains uncertain whether this type of gating is functional in K2P channels. The regulation of TWIK-related spinal cord K+ channel (TRESK) is different from the other K2P channels. Regulatory factors acting via the C-terminus are not known, instead channel activity is modified by the phosphorylation/dephosphorylation of the unusually long intracellular loop between the 2nd and 3rd transmembrane segments. These unique structural elements of the regulation lead us to examine channel gating at the bundle crossing region. Ba2+ was applied to the intracellular side of excised membrane patches and the characteristics of the channel block were determined. We compared the kinetics of t...Continue Reading

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Apr 30, 2016·The Journal of Biological Chemistry·Miklós LengyelPéter Enyedi
Jun 9, 2016·Molecular Pharmacology·María Isabel NiemeyerFrancisco V Sepúlveda

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Citations

Jun 11, 2020·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Anthony LewisSteve A N Goldstein
May 28, 2019·Chemical Biology & Drug Design·Daniel Şterbuleac
Apr 23, 2021·Journal of Molecular Biology·Andrew M NataleDaniel L Minor
Aug 28, 2021·International Journal of Molecular Sciences·Miklós LengyelGábor Czirják

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Methods Mentioned

BETA
in vitro transcription
transfection

Software Mentioned

Statistica
pCLAMP

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