PMID: 2108197Jan 1, 1990Paper

TRH raises cytosolic Ca2+ in human adenomatous lactotrophs

Journal of Endocrinological Investigation
A SpadaA Lania

Abstract

The effect of TRH on cytosolic free calcium concentrations, [Ca2+]i, was evaluated on cell suspensions obtained from 6 human PRL secreting pituitary adenomas. In these cells resting [Ca2+]i levels were variable (mean +/- SE; 103.8 +/- 6.5, n = 25); the addition of 100 nM TRH caused a marked [Ca2+]i rise within 20 sec., the peak values ranging from 200 to 437 nM (285 +/- 10.8 nM, n = 10). The transients induced by TRH were composed by a rapid increase, due to mobilization of calcium from intracellular stores, followed within a few seconds by a lower plateau which was due to stimulated influx from the extracellular space. In fact, when EGTA and verapamil were applied after TRH they caused the Ca2+ plateau to dissipate rapidly. The addition of 1 microM dopamine (DA) caused a substantial decrease of resting [Ca2+]i (about 10-30%) as well as an inhibition of the plateau phase induced by TRH. The effect of DA completely depended on extracellular Ca2+. The TRH-induced transients observed in adenomatous cells were quite similar in size and time course to those recorded in normal rat lactotrophs. As previously observed in rat lactotrophs, in adenomatous cells treatment with pertussis toxin (PTx, 1 microgram/ml for 4 h) was unable to aff...Continue Reading

References

Jun 2, 1979·Lancet·E A CowdenG M Teasdale
Mar 17, 1977·The New England Journal of Medicine·D L KleinbergA G Frantz
Jun 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·E WankeJ Meldolesi
Aug 1, 1983·The Journal of Clinical Endocrinology and Metabolism·M Le DafnietF Peillon
Oct 1, 1982·The Journal of Clinical Endocrinology and Metabolism·A SpadaG Giannattasio
Apr 6, 1981·Life Sciences·G GiannattasioA Spada

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