Tri-nucleotide receptors play a critical role in epithelial cell wound repair.

Purinergic Signalling
Ilene WeingerVickery Trinkaus-Randall

Abstract

The cornea plays a major role in the refraction of light to the retina. Therefore, the integrity and transparency of the corneal epithelium are critical to vision. Following injury, a combination of rapid signal transduction events and long-term cell migration are essential for wound closure. We have demonstrated previously that injury resulted in the release of nucleotides that induce the propagation of a Ca(2+) wave to neighboring cells. This suggests that nucleotides and their receptors are critical components of wound healing. Epidermal growth factor (EGF) and integrins also have been shown to play a role in injury. In this study, we demonstrate that pretreatment of cells with ATP and UTP inhibited the immediate wound response, while BzATP, ADP, and UDP did not affect this response. Tri-nucleotide pretreatment also reduced the EGF induced Ca(2+) response. Additionally, lower EC(50) concentrations of ATP and UTP triggered migration of cells that was enhanced further with EGF and was inhibited by the tripeptide, RGD. Results indicate that the desensitization induced by ATP and UTP was specific. While ADP and UDP cause a homologous desensitization of their own signal, they did not cause an inhibition of the wound response nor ...Continue Reading

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Citations

Apr 27, 2011·Purinergic Signalling·Edyta Gendaszewska-Darmach, Marta Kucharska
Dec 14, 2011·PloS One·Courtney MankusVickery Trinkaus-Randall
Aug 7, 2012·Seminars in Cancer Biology·Philip L LeopoldHongjun Wang
Oct 28, 2015·Experimental Eye Research·Yong-Soo ByunChoun-Ki Joo
Jun 20, 2008·Pharmacology & Therapeutics·Almudena CrookeJesús Pintor
May 11, 2007·Experimental Eye Research·Ilene BoucherVickery Trinkaus-Randall
Nov 7, 2012·Journal of Cellular Physiology·Cheryl Chi, Vickery Trinkaus-Randall
Jul 22, 2015·Progress in Retinal and Eye Research·Alexander V Ljubimov, Mehrnoosh Saghizadeh
Jul 18, 2015·Integrative Biology : Quantitative Biosciences From Nano to Macro·Kelsey E DerricksMatthew A Nugent
Mar 31, 2015·Trends in Cell Biology·Balázs Enyedi, Philipp Niethammer
Feb 15, 2014·Cell Adhesion & Migration·Paul Najm, Mirvat El-Sibai
Oct 8, 2013·The American Journal of Pathology·Amanuel KehasseVickery Trinkaus-Randall
Oct 19, 2016·Journal of Ocular Pharmacology and Therapeutics : the Official Journal of the Association for Ocular Pharmacology and Therapeutics·Martin S Minns, Vickery Trinkaus-Randall
Mar 17, 2010·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·Rongpei LanManjeri Venkatachalam
Mar 3, 2012·Radiation Research·Harish ShankaranMarianne B Sowa
Mar 29, 2014·American Journal of Physiology. Cell Physiology·Albert LeeVickery Trinkaus-Randall
Apr 25, 2014·American Journal of Physiology. Cell Physiology·Farid G El-SayedGary A Weisman
Aug 23, 2019·Biomaterials Science·Young Ju SonHyuk Sang Yoo
May 18, 2005·American Journal of Physiology. Cell Physiology·Silvia ChiffletSilvina Grasso
Apr 30, 2010·American Journal of Physiology. Cell Physiology·Ilene BoucherVickery Trinkaus-Randall
Oct 6, 2017·Biophysical Journal·Erica K ShannonM Shane Hutson
Aug 3, 2021·Analytical Cellular Pathology (Amsterdam)·Garrett RhodesVickery Trinkaus-Randall

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