PMID: 9182714Jun 1, 1997Paper

Triacsin C blocks de novo synthesis of glycerolipids and cholesterol esters but not recycling of fatty acid into phospholipid: evidence for functionally separate pools of acyl-CoA

The Biochemical Journal
R Ariel IgalR A Coleman

Abstract

The trafficking of acyl-CoAs within cells is poorly understood. In order to determine whether newly synthesized acyl-CoAs are equally available for the synthesis of all glycerolipids and cholesterol esters, we incubated human fibroblasts with [14C]oleate, [3H]arachidonate or [3H]glycerol in the presence or absence of triacsin C, a fungal metabolite that is a competitive inhibitor of acyl-CoA synthetase. Triacsin C inhibited de novo synthesis from glycerol of triacylglycerol, diacylglycerol and cholesterol esters by more than 93%, and the synthesis of phospholipid by 83%. However, the incorporation of oleate or arachidonate into phospholipids appeared to be relatively unimpaired when triacsin was present. Diacylglycerol acyltransferase and lysophosphatidylcholine acyltransferase had similar dependences on palmitoyl-CoA in both liver and fibroblasts; thus it did not appear that acyl-CoAs, when present at low concentrations, would be preferentially used to acylate lysophospholipids. We interpret these data to mean that, when fatty acid is not limiting, triacsin blocks the acylation of glycerol 3-phosphate and diacylglycerol, but not the reacylation of lysophospholipids. Two explanations are possible: (1) different acyl-CoA synthet...Continue Reading

Citations

Mar 1, 2012·European Journal of Medicinal Chemistry·Yunjeong KimKyeong-Ok Chang
Jan 26, 2007·Journal of Neurochemistry·Gwendolyn Barceló-CoblijnEric J Murphy
Apr 27, 2011·Cellular Microbiology·Manuel R GonzalezF Gisou van der Goot
Sep 15, 2004·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Theresa J Grove, Bruce D Sidell
Aug 11, 2015·Frontiers in Microbiology·Fengguang GuoGuan Zhu
Jul 15, 2015·European Journal of Pharmacology·Andrea DichlbergerWolfgang J Schneider
Aug 22, 2006·Current Biology : CB·Yadunanda KumarRaphael H Valdivia
Feb 1, 2012·Biochimica Et Biophysica Acta·Armen KhatchadourianDusica Maysinger
Dec 11, 2008·PLoS Biology·Mathias BellerBrian Oliver
Jun 4, 2016·Prostaglandins, Leukotrienes, and Essential Fatty Acids·D R SeegerE J Murphy
Aug 15, 2000·Annual Review of Nutrition·R A ColemanD M Muoio
May 14, 2014·Annual Review of Nutrition·Trisha J GrevengoedRosalind A Coleman
Oct 20, 2009·Nutrition and Cancer·Lynda M EvansRobert W Hardy
Nov 20, 2016·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Elizabeth P NewberryNicholas O Davidson
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Dec 3, 2017·Proceedings of the National Academy of Sciences of the United States of America·Yihui ShenWei Min
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Dec 28, 2012·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Delphine Ditlecadet, William R Driedzic

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