Tributyltin increases the expression of apoptosis- and adipogenesis-related genes in rat ovaries.

Clinical and Experimental Reproductive Medicine
Hyojin LeeHyunwon Yang

Abstract

Tributyltin (TBT), an endocrine disrupting chemical, has been reported to decrease ovarian function by causing apoptosis in the ovary, but the mechanism is not fully understood. Therefore, we examined whether TBT increases the expression of adipogenesis-related genes in the ovary and the increased expression of these genes is associated with apoptosis induction. Three-week-old Sprague-Dawley rats were orally administered TBT (1 or 10 mg/kg body weight) or sesame oil as a control for 7 days. The ovaries were obtained and weighed on day 8, and then they were fixed for terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) or frozen for RNA extraction. Using the total RNA of the ovaries, adipogenesis- and apoptosis-related genes were analyzed by real-time polymerase chain reaction (PCR). The ovarian weight was significantly decreased in rats administered 10 mg/kg TBT compared to that in control rats. As determined by the TUNEL assay, the number of apoptotic follicles in ovary was significantly increased in rats administered 10 mg/kg TBT. The real-time PCR results showed that the expression of adipogenesis-related genes such as PPARγ, aP2, CD36, and PEPCK was increased after TBT administration. In addition, apoptosis-...Continue Reading

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Citations

Jun 9, 2015·Reproductive Toxicology·Priscila L PodratzJones B Graceli
Jul 18, 2018·Journal of Toxicology and Environmental Health. Part B, Critical Reviews·Eduardo MerloJones B Graceli
Jun 1, 2013·Epidemiology and Infection·J Pakpoor, J Pakpoor
Mar 17, 2018·Frontiers in Endocrinology·Julia Fernandez Puñal de AraújoJones Bernardes Graceli
Jan 23, 2021·Toxicological Sciences : an Official Journal of the Society of Toxicology·Shaneann FrossBhupinder P S Vohra
May 10, 2019·Toxicology Letters·Eduardo MerloJones B Graceli

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Methods Mentioned

BETA
PCR

Related Concepts

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Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis

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