Tricalbins Are Required for Non-vesicular Ceramide Transport at ER-Golgi Contacts and Modulate Lipid Droplet Biogenesis

IScience
Atsuko IkedaKouichi Funato

Abstract

Lipid composition varies among organelles, and the distinct lipid composition is important for specific functions of each membrane. Lipid transport between organelles, which is critical for the maintenance of membrane lipid composition, occurs by either vesicular or non-vesicular mechanisms. In yeast, ceramide synthesized in the endoplasmic reticulum (ER) is transported to the Golgi apparatus where inositolphosphorylceramide (IPC) is formed. Here we show that a fraction of Tcb3p, a yeast tricalbin protein, localizes to ER-Golgi contact sites. Tcb3p and their homologs Tcb1p and Tcb2p are required for formation of ER-Golgi contacts and non-vesicular ceramide transport. Absence of Tcb1p, Tcb2p, and Tcb3p increases acylceramide synthesis and subsequent lipid droplet (LD) formation. As LD can sequester excess lipids, we propose that tricalbins act as regulators of ceramide transport at ER-Golgi contact sites to help reduce a potentially toxic accumulation of ceramides.

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Citations

Mar 13, 2021·Current Opinion in Cell Biology·Pablo LujanFelix Campelo
Apr 30, 2021·STAR Protocols·Atsuko IkedaKouichi Funato
May 2, 2021·Current Opinion in Cell Biology·Rossella VendittiMaria Antonietta De Matteis
May 3, 2021·The Journal of Biological Chemistry·Tiantian QianHaijia Yu
Apr 10, 2021··Atsuko IkedaKazuki Hanaoka

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Methods Mentioned

BETA
fluorescence microscopy
scraping

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