Tricarboxylic acid cycle activity regulates tomato root growth via effects on secondary cell wall production.

Plant Physiology
Margaretha J van der MerweAlisdair R Fernie

Abstract

Transgenic tomato (Solanum lycopersicum 'Moneymaker') plants independently expressing fragments of various genes encoding enzymes of the tricarboxylic acid cycle in antisense orientation have previously been characterized as exhibiting altered root growth. In this study, we evaluate the rates of respiration of roots from these lines in addition to determining their total dry weight accumulation. Given that these features were highly correlated, we decided to carry out an evaluation of the cell wall composition in the transformants that revealed a substantial reduction in cellulose. Since the bulk of cellulose is associated with the secondary cell walls in roots, we reasoned that the transformants most likely were deficient in secondary wall cellulose production. Consistent with these findings, cross-sections of the root collar (approximately 15 mm from the junction between root and stem) displayed reduced lignified secondary cell walls for the transformants. In contrast, cell and cell wall patterning displayed no differences in elongating cells close to the root tip. To further characterize the modified cell wall metabolism, we performed feeding experiments in which we incubated excised root tips in [U-(14)C]glucose in the pres...Continue Reading

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Citations

Jun 21, 2011·Trends in Plant Science·Wagner L AraújoAlisdair R Fernie
Mar 29, 2011·Phytochemistry·Wagner L AraújoAlisdair R Fernie
Dec 28, 2010·Journal of Plant Physiology·Joost T van DongenAlisdair R Fernie
Jul 17, 2013·The Plant Journal : for Cell and Molecular Biology·Romy SchmidtBernd Mueller-Roeber
Apr 1, 2016·Journal of Experimental Botany·Toshihiro ObataAlisdair R Fernie
Nov 20, 2019·Journal of Experimental Botany·Tiantian XieShengqing Shi
Jun 10, 2017·Frontiers in Plant Science·Clarice A MegguerFernando L Finger
Dec 29, 2019·BMC Plant Biology·Yongming LiuMoju Cao
Nov 19, 2019·Frontiers in Plant Science·Jelena BoeckxBart M Nicolaï

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