PMID: 8491772May 1, 1993Paper

Trichinella spiralis infected skeletal muscle cells arrest in G2/M and cease muscle gene expression

The Journal of Cell Biology
D P Jasmer

Abstract

Infection by Trichinella spiralis causes a variety of changes in skeletal muscle cells including the hypertrophy of nuclei and decreased expression of muscle specific proteins. Potential cellular processes leading to these changes were investigated. In synchronized muscle infections, [3H]thymidine was incorporated into infected cell nuclei from 2-5 days post infection. Labeled nuclei were stably integrated into the infected cell up to 60 days post infection and appear to originate from differentiated skeletal muscle nuclei present at the time of infection. These nuclei were further shown to contain a mean DNA content of approximately 4N, indicating that the [3H]thymidine uptake reflects DNA synthesis and subsequent long-term suspension of the infected cell in the cell cycle at G2/M. Associated with these changes, muscle specific gene transcripts were reduced to < 1- < 0.1% in the infected cell compared to normal muscle. Transcript levels of the muscle transcriptional regulatory factors myogenin, MyoD1, and Id were reduced to < 10, < 1, and increased approximately 250%, respectively, in the infected cell compared to normal muscle, indicating transcriptional inactivation of muscle genes. DNA synthesis in the infected cell may rep...Continue Reading

References

Feb 1, 1975·Experimental Parasitology·D DespommierL Turgeon
Jun 1, 1990·Differentiation; Research in Biological Diversity·S IujvidinD Yaffe
May 1, 1990·Experimental Parasitology·D P Jasmer
Jan 1, 1985·The Journal of Cell Biology·A M TassinM Bornens
Sep 1, 1967·Proceedings of the National Academy of Sciences of the United States of America·M Fogel, V Defendi
Jan 15, 1968·International Journal of Cancer. Journal International Du Cancer·H H LeeJ D Ebert
May 1, 1966·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·D M Maeir, H Zaiman
Jan 1, 1983·Medicine and Science in Sports and Exercise·B M Carlson, J A Faulkner

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Citations

Dec 1, 1993·Plant Molecular Biology·P C Sijmons
Oct 30, 1996·Molecular and Biochemical Parasitology·B ConnollyD F Smith
Oct 1, 1996·Molecular and Biochemical Parasitology·L E PerrymanM J Arrowood
Jan 1, 1994·Parasitology Today·A NiebelM Van Montagu
Mar 9, 2004·Molecular and Biochemical Parasitology·Daniel GareBernadette Connolly
Mar 7, 2013·Veterinary Parasitology·Magdalena Dabrowska
Feb 26, 2013·Veterinary Parasitology·Z WuY Takahashi
Jun 1, 1996·Molecular and Biochemical Parasitology·D K VassilatisL H Van der Ploeg
Mar 13, 1998·Molecular and Biochemical Parasitology·S R ArdenM E Selkirk
Oct 17, 2006·Parasitology Today·D D Despommier
Dec 2, 2000·Veterinary Parasitology·W Kociecka
Aug 6, 1996·Proceedings of the National Academy of Sciences of the United States of America·D P JasmerT C McGuire
Apr 19, 2008·DNA and Cell Biology·Ma-feng LiuMing-yuan Liu
Dec 29, 2000·The Journal of Eukaryotic Microbiology·M ScanlonG J Leitch
Jan 1, 1997·Brain Pathology·A L Taratuto, S M Venturiello
Nov 10, 2001·Annual Review of Phytopathology·Eric L DavisPierre Abad
Jul 31, 2002·Annual Review of Phytopathology·Godelieve Gheysen, Carmen Fenoll
Oct 7, 2003·Annual Review of Phytopathology·Douglas P JasmerGeert Smant
Aug 20, 2008·Parasites & Vectors·Zhiliang WuYuzo Takahashi
Jan 13, 1997·The Journal of Cell Biology·E M TanakaJ P Brockes
Sep 24, 2004·Molecular and Biochemical Parasitology·Makedonka MitrevaJames P McCarter
May 3, 2011·Veterinary Parasitology·Yun-Jeong KangHee-Jae Cha
Dec 17, 2008·Veterinary Parasitology·M V FabreJ A Appleton
Dec 2, 2008·Veterinary Parasitology·X L WangM Y Liu
Nov 4, 2006·Molecular and Biochemical Parasitology·Mark W RobinsonBernadette Connolly
Nov 11, 2008·International Journal for Parasitology·David B GuilianoMurray E Selkirk

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