Trichomonas vaginalis NTPDase inhibited by lycorine modulates the parasite-neutrophil interaction

Parasitology Research
Brenda Petró-SilveiraTiana Tasca

Abstract

Lycorine is an Amaryllidaceae alkaloid that presents anti-Trichomonas vaginalis activity. T. vaginalis causes trichomoniasis, the most common non-viral sexually transmitted infection. The modulation of T. vaginalis purinergic signaling through the ectonucleotidases, nucleoside triphosphate diphosphohydrolase (NTPDase), and ecto-5'-nucleotidase represents new targets for combating the parasite. With this knowledge, the aim of this study was to investigate whether NTPDase and ecto-5'-nucleotidase inhibition by lycorine could lead to extracellular ATP accumulation. Moreover, the lycorine effect on the reactive oxygen species (ROS) production by neutrophils and parasites was evaluated as well as the alkaloid toxicity. The metabolism of purines was assessed by HPLC. ROS production was measured by flow cytometry. Cytotoxicity against epithelial vaginal cells and fibroblasts was tested, as well as the hemolytic effect of lycorine and its in vivo toxicity in Galleria mellonella larvae. Our findings showed that lycorine caused ATP accumulation due to NTPDase inhibition. The alkaloid did not affect the ROS production by T. vaginalis; however, it increased ROS levels in neutrophils incubated with lycorine-treated trophozoites. Lycorine wa...Continue Reading

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Citations

Sep 11, 2020·BMC Complementary Medicine and Therapies·Mendel FriedmanKirkwood M Land
Sep 6, 2020·Current Topics in Medicinal Chemistry·Micheli Ferla, Tiana Tasca

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Methods Mentioned

BETA
NMR
flow cytometry

Software Mentioned

BD
GraphPad Prism6
LC Solution

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