Trichostatin a enhances gap junctional intercellular communication in primary cultures of adult rat hepatocytes

Toxicological Sciences : an Official Journal of the Society of Toxicology
Mathieu VinkenV Rogiers

Abstract

The effects of histone deacetylase inhibitor Trichostatin A (TSA) on connexin (Cx) expression and gap junctional intercellular communication (GJIC) were investigated in primary cultures of adult rat hepatocytes. GJIC was monitored by using the scrape-loading/dye transfer method. Immunoblotting and immunocytochemistry were used to investigate Cx protein levels and localization. Cx gene expression was studied by means of quantitative reverse transcriptase-polymerase chain reaction. TSA increased Cx32 protein levels and affected negatively the Cx26 protein levels. The latter was preferentially located in the cytosol of cultured cells. TSA also promoted the appearance of Cx43 in the nuclear compartment of primary cultured hepatocytes. Overall, this resulted in enhanced GJIC activity. It is important to note that the time of onset of TSA treatment was crucial for the extent of its outcome and that the effects of TSA on Cx protein levels occurred independently of transcriptional changes. TSA differentially affects Cx proteins in primary rat hepatocyte cultures, suggesting distinct regulation and/or distinct roles of the different Cx species in the control of hepatic homeostasis. TSA enhances GJIC between primary cultured rat hepatocy...Continue Reading

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Citations

Mar 30, 2011·Investigational New Drugs·Joanna Edyta FraczekVera Rogiers
Dec 5, 2009·Cellular and Molecular Life Sciences : CMLS·Mathieu VinkenVera Rogiers
Oct 18, 2011·Toxicological Sciences : an Official Journal of the Society of Toxicology·Mathieu VinkenVera Rogiers
Feb 2, 2013·Drug Metabolism Reviews·Eva RamboerMathieu Vinken
Jul 1, 2006·Critical Reviews in Toxicology·Mathieu VinkenTamara Vanhaecke
Sep 7, 2012·Cell Communication & Adhesion·Mathieu VinkenVera Rogiers
Nov 14, 2015·Biochimica Et Biophysica Acta·Mathieu Vinken
Jan 21, 2012·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Mathieu VinkenVera Rogiers
Apr 26, 2011·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Jennifer BolleynTamara Vanhaecke
Nov 26, 2010·European Journal of Cell Biology·Sachie YamajiMarta Varela-Rey
Oct 12, 2010·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Tom HenkensTamara Vanhaecke
Oct 1, 2015·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Michaël MaesMathieu Vinken
Sep 20, 2008·Biochimica Et Biophysica Acta·Mathieu VinkenVera Rogiers
Jul 9, 2009·Journal of Cellular and Molecular Medicine·Fraczek JoannaRogiers Vera
Dec 7, 2007·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Mathieu VinkenVera Rogiers
Jun 22, 2010·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Aki KaimoriAyman A Koteish
May 31, 2013·Biology of the Cell·Catheleyne D'hondtGeert Bultynck
Mar 24, 2016·Cell Communication & Adhesion·Joost WillebrordsMathieu Vinken
Jun 19, 2015·Cellular and Molecular Life Sciences : CMLS·Michaël MaesMathieu Vinken
Oct 21, 2011·World Journal of Gastrointestinal Pathophysiology·Mathieu Vinken
Aug 16, 2017·Scientific Reports·Joost WillebrordsMathieu Vinken
Sep 29, 2019·World Journal of Gastroenterology : WJG·Huan LiXiao-Ming Liu
May 25, 2016·Journal of Gastroenterology and Hepatology·Wei-Lun TsaiShih-Chieh Hung
Sep 11, 2020·International Journal of Molecular Sciences·Axelle CooremanMathieu Vinken
Aug 28, 2021·International Journal of Molecular Sciences·Iva SovadinováPavel Babica

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