PMID: 11922434Apr 2, 2002Paper

Triclabendazole-resistant Fasciola hepatica: beta-tubulin and response to in vitro treatment with triclabendazole

Parasitology
Mark W RobinsonI Fairweather

Abstract

Resistance in Fasciola hepatica to triclabendazole ('Fasinex') has emerged in several countries. Benzimidazole resistance in parasitic nematodes has been linked to a single amino acid substitution (phenylalanine to tyrosine) at position 200 on the beta-tubulin molecule. Sequencing of beta-tubulin cDNAs from triclabendazole-susceptible and triclabendazole-resistant flukes revealed no amino acid differences between their respective primary amino acid sequences. In order to investigate the mechanism of triclabendazole resistance, triclabendazole-susceptible and triclabendazole-resistant flukes were incubated in vitro with triclabendazole sulphoxide (50 microg/ml). Scanning and transmission electron microscopy revealed extensive damage to the tegument of triclabendazole-susceptible F. hepatica, whereas triclabendazole-resistant flukes showed only localized and relatively minor disruption of the tegument covering the spines. Immunocytochemical studies, using an anti-tubulin antibody, showed that tubulin organization was disrupted in the tegument of triclabendazole-susceptible flukes. No such disruption was evident in triclabendazole-resistant F. hepatica. The significance of these findings is discussed with regard to the mechanism o...Continue Reading

Citations

May 29, 2007·Parasitology Research·M P Vara-Del RíoF A Rojo-Vázquez
Nov 19, 2009·Parasitology Research·Maeve McConvilleIan Fairweather
Aug 3, 2010·Parasitology Research·Emma ChambersDavid J Timson
Oct 29, 2013·International Journal for Parasitology·Marc-Aurel FuchsElizabeth M Hoey
Apr 16, 2009·Journal of Helminthology·I Fairweather
Aug 19, 2009·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Mark W Robinson, John P Dalton
Sep 9, 2016·Journal of Parasitic Diseases : Official Organ of the Indian Society for Parasitology·Mohammad Moazeni, Ali Asghar Khademolhoseini
Nov 26, 2005·Expert Opinion on Investigational Drugs·Jennifer KeiserJürg Utzinger
Apr 24, 2012·Veterinary Parasitology·F A Rojo-VázquezM Martínez-Valladares
Jan 14, 2011·Veterinary Parasitology·I Fairweather
Nov 26, 2008·Veterinary Parasitology·L HalfertyI Fairweather
Apr 3, 2007·Experimental and Molecular Pathology·G P BrennanP M Brophy
Nov 23, 2006·Veterinary Parasitology·Ahmed G HegaziHatem A Shalaby
Sep 15, 2004·Trends in Parasitology·Adrian J WolstenholmeNicholas C Sangster
Apr 7, 2016·Trends in Parasitology·Jane M KelleyTerry W Spithill
Mar 29, 2008·Molecular and Biochemical Parasitology·Louise A RyanDavid Johnston
Oct 13, 2010·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·D TeofanovaA Trudgett

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