Tricorn protease (TRI) interacting factor 1 from Thermoplasma acidophilum is a proline iminopeptidase

FEBS Letters
T TamuraW Baumeister

Abstract

Tricorn protease (TRI), a high molecular mass complex from the archaeon T. acidophilum, forms the core of a modular proteolytic system; upon interacting with low molecular mass factors intrinsic activities are enhanced and novel activities are generated. Here we characterize the first factor, F1, which turns out to be homologous with several bacterial proline iminopeptidases (PIPs). Surprisingly, it cleaves not only typical PIP substrates such as H-Pro-AMC, but a wide spectrum of amino acid substrates and several peptide substrates without a proline at the N-terminus. The pip gene encodes a 293 amino acid residue protein with a molecular mass of 33,487 Da. By means of site-directed mutagenesis we identified Ser105 and His271 as the active site nucleophile and proton donor, respectively. Experiments with inactive mutant PIPs indicate that the activities elicited by interacting with TRI are contributed by PIP.

References

Apr 1, 1992·Protein Engineering·D L OllisJ Schrag
Jan 1, 1990·Methods in Enzymology·F W StudierJ W Dubendorff
Jun 1, 1994·FEMS Microbiology Letters·E G Dudley, J L Steele

❮ Previous
Next ❯

Citations

Nov 14, 1997·Trends in Biochemical Sciences·A LupasW Baumeister
Feb 8, 2008·The Journal of Biological Chemistry·Longsheng LaiGordon L Amidon
Feb 16, 2010·Applied and Environmental Microbiology·Khalid Ibrahim SallamTomohiro Tamura
Nov 20, 2002·Biological Chemistry·Hans BrandstetterRobert Huber
Mar 31, 2004·Biotechnology and Bioengineering·Nobutaka Nakashima, Tomohiro Tamura
May 9, 2012·Journal of Bioscience and Bioengineering·Nobutaka Nakashima, Tomohiro Tamura
Jan 28, 2006·FEMS Microbiology Reviews·Rosana E De CastroJorge J Sánchez
Sep 2, 1999·Current Biology : CB·T Yao, R E Cohen
Jul 11, 1998·Molecular Cell·J WalzA J Koster
Jan 29, 2005·Chembiochem : a European Journal of Chemical Biology·Michael GrollRobert Huber
Jul 5, 2005·The Journal of Biological Chemistry·Peter GoettigJeong-Sun Kim
Dec 1, 2018·Emerging Topics in Life Sciences·Julie A Maupin-Furlow

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.