Triflavin inhibits platelet-induced vasoconstriction in de-endothelialized aorta

Arteriosclerosis, Thrombosis, and Vascular Biology
J R SheuT F Huang

Abstract

Triflavin, a 7.5-kD cysteine-rich polypeptide purified from Trimeresurus favoviridis snake venom, belongs to a family of Arg-Gly-Asp-(RGD)-containing peptides, termed disintegrins. In this study, aggregating human platelets dose-dependently induced vasoconstriction in de-endothelialized isolated rat thoracic aortas. At 5x10(7) cells per milliliter, platelets induced a peak tension averaging 65 +/- 7.2% of the tension induced by phenylephrine (10 mumol/L). The relative effectiveness of RGD-containing peptides (including venom peptides triflavin and trigramin, small RGD synthetic peptides Gly-Arg-Gly-Asp-Ser [GRGDS], Gly-Arg-Gly-Asp-Phe [GRGDF], and Gly-Arg-Gly-Asp-Ser-Pro-Lys [GRGDSPK]) was examined by testing the inhibitory effect on aggregating platelet-induced vasoconstriction in de-endothelialized aorta. Triflavin (1 mumol/L) significantly inhibited the platelet-induced vasoconstriction, whereas neither trigramin (10 mumol/L) nor small RGD peptides (2 mmol/L) (i.e., GRGDS, GRGDF, and GRGDSPK) showed any significant effect. The release of serotonin and the formation of thromboxane A2 from aggregating platelets were both significantly inhibited by triflavin (2 mumol/L), whereas trigramin and small RGD-containing peptides showe...Continue Reading

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Citations

May 10, 2001·International Journal of Hematology·J R SheuM H Yen
May 31, 2003·International Journal of Hematology·Joen R SheuMao H Yen
Jul 23, 2003·Anesthesia and Analgesia·Greg Stratmann, George A Gregory
Aug 21, 2002·Biological & Pharmaceutical Bulletin·George HsiaoJoen-Rong Sheu
Sep 22, 2007·The Journal of Pharmacy and Pharmacology·Chi-Feng PanJoen-Rong Sheu
Sep 11, 2020·International Journal of Molecular Sciences·Augusto Martins LimaNikolaos Stergiopulos
Apr 3, 2001·The Journal of Laboratory and Clinical Medicine·J R SheuC H Lin

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