PMID: 2110826Jan 23, 1990Paper

Trifluoperazine binding to porcine brain calmodulin and skeletal muscle troponin C

Biochemistry
L R MassomH W Jarrett

Abstract

Binding of trifluoperazine (TFP), a phenothiazine tranquilizer, to porcine brain calmodulin (CaM) and rabbit skeletal muscle troponin C (Tn C) was measured by an automated high-performance liquid chromatography binding assay using a molecular sieving column; 10 micrograms of either protein per injection is sufficient for determining TFP binding, and results are comparable to those obtained by equilibrium dialysis. Very little binding was observed to either protein in the absence of Ca2+ while in the presence of Ca2+ both proteins bind 4 equiv of TFP. Other characteristics of TFP binding however are different for each protein. For CaM, half-maximal binding occurs at 5.8 microM TFP, the Hill coefficient is 0.82, and the fit of the data to the Scatchard equation is consistent with four independent TFP-binding sites. Binding of one melittin displaces two TFP from CaM. Thus, there are two recognizable classes of TFP-binding sites: those that are displaced by melittin and those that are not. TFP causes an increase in the Ca2+ affinity of CaM, and three Ca2+ must be bound to CaM for TFP binding to occur. The studies also yielded a measure of the intrinsic affinity of three of CaM's Ca2(+)-binding sites that is in agreement with previo...Continue Reading

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Citations

Oct 1, 1994·Journal of Neurobiology·X JianJ T Schmidt
Mar 1, 1995·Neurochemical Research·M SitgesL M Chiu
Oct 1, 1993·Journal of Bioenergetics and Biomembranes·D C Wooten, R A Dilley
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Sep 6, 2003·Proceedings of the National Academy of Sciences of the United States of America·Bijay S Jaiswal, Marco Conti
Jun 5, 2010·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Erika KovacsKároly Liliom
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Jan 17, 2017·Journal of Cellular Biochemistry·Romone M FancyYuhua Song

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