PMID: 11342176May 9, 2001Paper

Trifluoperazine induces domain formation in zwitterionic phosphatidylcholine but not in charged phosphatidylglycerol bilayers

Biochimica Et Biophysica Acta
A B HendrichK Michalak

Abstract

The interaction of trifluoperazine with the zwitterionic lipids dipalmitoylphosphatidylcholine and dimyristoylphosphatidylcholine and with anionic dimyristoylphosphatidylglycerol was studied by means of microcalorimetry and fluorescence spectroscopy. Intercalation of drug molecules into the lipid bilayers was confirmed by the observed differential scanning calorimetry peak broadening and the decrease in chain-melting temperatures. For trifluoperazine:lipid mole ratios higher than 0.4 and 0.6 (for dipalmitoylphosphatidylcholine and dimyristoylphosphatidylcholine, respectively) the deconvolution of transition profiles into two Gaussian components was possible, which suggests phase separation in the studied mixtures. Deconvolution of the thermograms was not possible for any of the drug:dimyristoylphosphatidylglycerol mole ratios studied. To confirm the existence of phase separation in trifluoperazine-phosphatidylcholine mixtures fluorescence spectroscopy experiments were performed using Laurdan as a probe. From the generalised polarisation versus excitation wavelength dependences, recorded at different temperatures, we conclude that a phase separation occurs in the gel state of the studied trifluoperazine-phosphatidylcholine mixtu...Continue Reading

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Citations

Apr 3, 2004·Biophysical Chemistry·A A HidalgoO N Oliveira
Mar 5, 2004·European Journal of Pharmaceutical Sciences : Official Journal of the European Federation for Pharmaceutical Sciences·Andrzej PołaMasami Kawase
Apr 25, 2003·Biochemical and Biophysical Research Communications·Andrzej B HendrichKrystyna Michalak
Jul 20, 2002·Biophysical Chemistry·Andrzej B HendrichKrystyna Michalak
Jul 20, 2002·European Journal of Pharmaceutical Sciences : Official Journal of the European Federation for Pharmaceutical Sciences·Andrzej Boguslaw HendrichKrystyna Michalak
Oct 2, 2012·American Journal of Respiratory and Critical Care Medicine·Chi-Tai YehChi-Ying F Huang
Oct 22, 2011·Expert Opinion on Investigational Drugs·Sadhna Sharma, Amandeep Singh
Jan 15, 2016·The Journal of Membrane Biology·Mikhail V DubininKonstantin N Belosludtsev
Feb 13, 2007·Colloids and Surfaces. B, Biointerfaces·Konrad WerońskiJosefina Prat
Sep 24, 2004·Biophysical Chemistry·Cristina LariosIsabel Haro
Jun 1, 2006·Bioorganic & Medicinal Chemistry·Andrzej B HendrichKrystyna Michalak
May 19, 2006·European Journal of Pharmaceutical Sciences : Official Journal of the European Federation for Pharmaceutical Sciences·Soon Young ShinYoung Han Lee
Feb 14, 2004·Advances in Colloid and Interface Science·Valery Kochev, Miroslav Karabaliev
Sep 26, 2013·Biochimica Et Biophysica Acta·Olga WesołowskaKrystyna Michalak
Jun 17, 2009·Biochimica Et Biophysica Acta·Olga WesołowskaKrystyna Michalak
Feb 22, 2013·The Journal of Physical Chemistry. B·Katarzyna Cieślik-BoczulaAleksander Koll
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Sep 29, 2006·The Journal of Physical Chemistry. B·A A HidalgoO N Oliveira
Jul 30, 2021·The Journal of Biological Chemistry·Anne Sofie Busk HeitmannJesper Nylandsted

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