Trifluoroacetic acid as excipient destabilizes melittin causing the selective aggregation of melittin within the centrin-melittin-trifluoroacetic acid complex

Structural Dynamics
Belinda Pastrana-RiosJorge Santiago

Abstract

Trifluoroacetic acid (TFA) may be the cause of the bottleneck in high resolution structure determination for protein-peptide complexes. Fragment based drug design often involves the use of synthetic peptides which contain TFA (excipient). Our goal was to explore the effects of this excipient on a model complex: centrin-melittin-TFA. We performed Fourier transform infrared, two-dimensional infrared correlation spectroscopies and spectral simulations to analyze the amide I'/I'* band for the components and the ternary complex. Melittin (MLT) was observed to have increased helicity upon its interaction with centrin, followed by the thermally induced aggregation of MLT within the ternary complex in the TFA presence.

References

Jan 1, 1976·Annual Review of Biochemistry·R H Kretsinger
May 1, 1989·The Journal of Cell Biology·M A Sanders, J L Salisbury
Sep 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·M KataokaD M Engelman
Jan 1, 1983·The Biochemical Journal·M ComteJ A Cox
Jan 1, 1993·Progress in Biophysics and Molecular Biology·J L ArrondoF M Goñi
May 2, 2000·FEBS Letters·I DurusselJ A Cox
Aug 2, 2001·Biochemistry·B Pastrana-Rios
May 30, 2002·Biochemistry·Belinda Pastrana-RiosJeffrey L Salisbury
Jul 5, 2003·Journal of Molecular Biology·Haitao Hu, Walter J Chazin
Jan 19, 2005·Biochemistry·Jos A CoxConstantin T Craescu
Feb 16, 2005·Biochemistry·Mildred OrtizBelinda Pastrana-Rios
Jun 21, 2005·Molecular and Cellular Biology·Ryotaro NishiFumio Hanaoka
Aug 19, 2007·Biochimica Et Biophysica Acta·Andreas Barth
Aug 19, 2007·Journal of Cellular Physiology·Jeffrey L Salisbury
Oct 13, 2011·Proteins·Liliana Del Valle SosaBelinda Pastrana-Ríos
Jan 26, 2013·Biochemistry·Belinda Pastrana-RíosMara Colón

❮ Previous
Next ❯

Citations

Dec 17, 2020·Analytical Chemistry·Belinda PastranaDimiter S Dimitrov
Dec 21, 2018·The Journal of Physical Chemistry. B·Lisa S RamirezAlexander Shekhtman

❮ Previous
Next ❯

Methods Mentioned

BETA
circular dichroism

Software Mentioned

MATLAB

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.