Triggered radiosensitizer delivery using thermosensitive liposomes and hyperthermia improves efficacy of radiotherapy: An in vitro proof of concept study

PloS One
Helena C BesseRoel Deckers

Abstract

To increase the efficacy of chemoradiation and decrease its toxicity in normal tissue, a new concept is proposed, local radiosensitizer delivery, which combines triggered release of a radiosensitizer from thermosensitive liposomes with local hyperthermia and radiotherapy. Here, key aspects of this concept were investigated in vitro I) the effect of hyperthermia on the enhancement of radiotherapy by ThermoDox (thermosensitive liposome containing doxorubicin), II) the concentration dependence of the radiosensitizing effect of doxorubicin and III) the sequence of doxorubicin, hyperthermia and radiotherapy maximizing the radiosensitizing effect. Survival of HT1080 (human fibrosarcoma) cells was measured after exposure to ThermoDox or doxorubicin for 60 minutes, at 37 or 43°C, with or without irradiation. Furthermore, cell survival was measured for cells exposed to different doxorubicin concentrations and radiation doses. Finally, cell survival was measured after applying doxorubicin and/or hyperthermia before or after irradiation. Cell survival was measured by clonogenic assay. In addition, DNA damage was assessed by γH2AX staining. Exposure of cells to doxorubicin at 37°C resulted in cell death, but exposure to ThermoDox at 37°C d...Continue Reading

References

Mar 1, 1975·Proceedings of the National Academy of Sciences of the United States of America·G M HahnI Har-Kedar
May 1, 1977·Radiology·W C DeweyL E Gerweck
Jan 1, 1990·International Journal of Radiation Biology·J A Bonner, T S Lawrence
Apr 1, 1991·International Journal of Radiation Oncology, Biology, Physics·L Roizin-Towle, J P Pirro
May 1, 1990·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·E E Vokes, R R Weichselbaum
Jan 1, 1982·International Journal of Radiation Oncology, Biology, Physics·L J PetersG H Fletcher
Jan 7, 1998·International Journal of Radiation Biology·P J van de VaartA C Begg
Apr 17, 2001·International Journal of Radiation Biology·H H Kampinga, E Dikomey
Jul 6, 2002·Critical Reviews in Oncology/hematology·Bert HildebrandtHanno Riess
Jan 2, 2003·Head & Neck·Luka MilasKian K Ang
May 13, 2003·Clinical Pharmacokinetics·Alberto GabizonYechezkel Barenholz
Aug 7, 2003·The Lancet Oncology·Henry Q XiongJaffer A Ajani
Mar 5, 2004·Annals of Oncology : Official Journal of the European Society for Medical Oncology·M E R O'BrienUNKNOWN CAELYX Breast Cancer Study Group
Oct 6, 2005·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Kimberley M LaginhaTheresa M Allen
Jan 30, 2007·Nature Clinical Practice. Oncology·Tanguy Y SeiwertEverett E Vokes
Apr 5, 2007·Nature Protocols·Nicolaas A P FrankenChris van Bree
May 12, 2007·Clinical Oncology : a Journal of the Royal College of Radiologists·M R Horsman, J Overgaard
Aug 31, 2007·International Journal of Pharmaceutics·Janet WooMarcel B Bally
Sep 16, 2008·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·Rolf D Issels
Jan 17, 2009·Nature Reviews. Cancer·Gillian C BarnettNeil G Burnet
Jul 6, 2010·International Journal of Hyperthermia : the Official Journal of European Society for Hyperthermic Oncology, North American Hyperthermia Group·Pavel S YarmolenkoMark W Dewhirst
May 11, 2011·Proceedings of the National Academy of Sciences of the United States of America·Przemek M KrawczykJacob A Aten
Sep 10, 2011·Journal of the National Cancer Institute·Walter J CurranJames D Cox
Sep 29, 2011·Journal of Controlled Release : Official Journal of the Controlled Release Society·Twan LammersGert Storm
Jan 3, 2012·Journal of Controlled Release : Official Journal of the Controlled Release Society·Ashish RanjanMatthew R Dreher
Nov 17, 2012·International Journal of Hyperthermia : the Official Journal of European Society for Hyperthermic Oncology, North American Hyperthermia Group·Robert M StaruchRajiv Chopra
Mar 26, 2013·Journal of Controlled Release : Official Journal of the Controlled Release Society·Li LiGerben A Koning
Apr 16, 2013·Journal of Controlled Release : Official Journal of the Controlled Release Society·Terence Ta, Tyrone M Porter
Nov 25, 2014·Pharmaceutical Research·Chantal Al SabbaghElias Fattal
Dec 18, 2015·International Journal of Oncology·Shohei MiyanagaTetsuo Ohta
Jun 3, 2016·CA: a Cancer Journal for Clinicians·Kimberly D MillerAhmedin Jemal

❮ Previous
Next ❯

Citations

Oct 18, 2019·Molecular and Clinical Oncology·Agata MikolajczykTanja Khosrawipour

❮ Previous
Next ❯

Methods Mentioned

BETA
X-ray
flow cytometry

Software Mentioned

ThermoDox
GraphPad Prism
SPSS
FlowJo

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

International Journal of Hyperthermia : the Official Journal of European Society for Hyperthermic Oncology, North American Hyperthermia Group
Robert M StaruchRajiv Chopra
© 2022 Meta ULC. All rights reserved