PMID: 6172541Jan 1, 1982Paper

Triggering of affinity-enriched B cells. Analysis of B cell stimulation by antigen-specific helper factor or lipopolysaccharide. I. Dissection into proliferative and differentiative signals

The Journal of Experimental Medicine
M A JaworskiE Diener

Abstract

Proliferative and differentiative signals controlling the in vitro IgM response by unprimed, affinity-enriched B cells were studied using conditions under which as few as 2,000 B cells stimulated by antigen-specific, Ia-positive, allogeneically restricted, T cell-derived helper factor (Hf) or the polyclonal activator lipopolysaccharide (LPS) yielded on the average 400 antibody-forming cells (AFC) by direct plaque assay. Antigen alone induces neither B cell proliferation nor differentiation into AFC. Proliferation but not differentiation into AFC is induced when affinity-enriched B cells are cultured in the presence of Ag and Hf or LPS but in the absence of nonantigen-specific, radioresistant, accessory (A) cells. For the induction of a complete Hf- or LPS-mediated AFC response, cultures must be reconstituted with A cells or the secretory product(s) of these cells. The antigen-specific response depends strictly on the presence of the Hf specific for the relevant antigen, regardless of the cell cycle state of cooperating B cells. The differentiative signal from A cells is due, at least in part, to the presence of a Thy-1.2-bearing population of cells. In the case of the LPS-mediated, but not the Hf-mediated response. A cells can ...Continue Reading

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Citations

Sep 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·M L JelachichS K Pierce
Oct 1, 1983·Scandinavian Journal of Immunology·C Fernandez, E Severinson
Apr 1, 1984·Immunological Reviews·M HowardW E Paul
Dec 1, 1982·Immunology Today·R F Ashman

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