Triggering the activation of Activin A type II receptor in human adipose stem cells towards tenogenic commitment using mechanomagnetic stimulation

Nanomedicine : Nanotechnology, Biology, and Medicine
Ana I GonçalvesA J El Haj

Abstract

Stem cell therapies hold potential to stimulate tendon regeneration and homeostasis, which is maintained in response to the native mechanical environment. Activins are members of the mechano-responsive TGF-β superfamily that participates in the regulation of several downstream biological processes. Mechanosensitive membrane receptors such as activin can be activated in different types of stem cells via magnetic nanoparticles (MNPs) through remote magnetic actuation resulting in cell differentiation. In this work, we target the Activin receptor type IIA (ActRIIA) in human adipose stem cells (hASCs), using anti-ActRIIA functionalized MNPs, externally activated through a oscillating magnetic bioreactor. Upon activation, the phosphorylation of Smad2/3 is induced allowing translocation of the complex to the nucleus, regulating tenogenic transcriptional responses. Our study demonstrates the potential remote activation of MNPs tagged hASCs to trigger the Activin receptor leading to tenogenic differentiation. These results may provide insights toward tendon regeneration therapies.

Citations

May 26, 2018·Biomedical Materials·Ana I GonçalvesManuela E Gomes
Sep 18, 2020·International Journal of Molecular Sciences·Maria Rita CiteroniBarbara Barboni
May 5, 2020·Frontiers in Bioengineering and Biotechnology·Abdel Rahman Abdel Fattah, Adrian Ranga
Nov 8, 2018·International Journal of Molecular Sciences·Chiara ArgentatiSabata Martino
Oct 9, 2020·Stem Cells International·Renwang ShengJialin Chen
Jan 8, 2021·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Ana F AlmeidaManuela E Gomes
Aug 8, 2021·Cells·Hareklea MarkidesAlicia J El Haj
Aug 17, 2021·Frontiers in Medicine·Zhi Jie LiYou Lang Zhou
Oct 22, 2020·ACS Synthetic Biology·Paul A HumphreysSusan J Kimber
Nov 7, 2021·Small·Miriam FilippiRobert K Katzschmann

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