Triglyceride accumulation in long-term cultures of adult rat hepatocytes by chronic exposure to Aroclor 1254

Journal of Toxicology and Environmental Health
T Mendoza-FigueroaM L López

Abstract

The effect of chronic exposure to micromolar concentrations of Aroclor 1254 (Aro) on the hepatic lipid metabolism was studied in long-term cultures of adult rat hepatocytes. Hepatocytes were cocultivated with mitomycin C-treated 3T3 cells and exposed for 2 wk to Aroclor 1254 concentrations ranging from 0.01 to 20 micrograms/ml. The Aro-exposed cultures showed intracytoplasmic lipid droplets and a maximum increase of 55% in the triglyceride (TG) content and of 4.4-fold in the cytochrome P-450 content. Labeling studies with [14C]acetic and [14C]oleic acid showed no changes in the uptake of fatty acid and TG precursors by the Aro-treated cultures; the synthesis of cellular lipids from [14C]acetic acid was slightly inhibited by Aroclor 1254, but that from [14C]oleic acid was increased, specially for TG (37%). The secretion of total lipids and TG was 2.1- and 2.7-fold lower, respectively, in the cultures treated with 20 micrograms/ml of Aroclor 1254, resulting in an increase of 1.9-fold in the intracellular content of TG. The synthesis of cellular proteins labeled with [3H]leucine was unchanged in the Aro-treated cultures, but the secretion of exportable proteins was 1.7-fold lower in the cultures treated with 20 micrograms/ml of Ar...Continue Reading

References

Nov 1, 1977·Clinical Pharmacology and Therapeutics·A P Alvares, A Kappas
Jun 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·W StremmelP D Berk
Mar 1, 1985·Bulletin of Environmental Contamination and Toxicology·J W Carter
Aug 1, 1985·Fundamental and Applied Toxicology : Official Journal of the Society of Toxicology·R L Jansing, W Shain
Mar 1, 1973·Toxicology and Applied Pharmacology·J V BrucknerH H Cornish
Jan 1, 1980·Methods in Cell Biology·J G Rheinwald
Dec 1, 1984·Journal of Applied Toxicology : JAT·T Mendoza-Figueroa
Mar 1, 1980·Archives of Environmental Health·H Kodama, H Ota

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Citations

Jan 1, 1992·Biology of the Cell·A R Rincón-SánchezT Mendoza-Figueroa
Jan 1, 1996·Comparative Biochemistry and Physiology. Part C, Pharmacology, Toxicology & Endocrinology·M DuboisP Kremers
Apr 1, 1995·Toxicology in Vitro : an International Journal Published in Association with BIBRA·A RotemM L Yarmush
Nov 1, 1994·Journal of Invertebrate Pathology·D M HarperC L Reinisch
Dec 1, 2012·International Journal of Toxicology·J Phillip KaiserScott C Wesselkamper
Nov 2, 1999·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·S N BhatiaM Toner
Feb 5, 1991·Biochimica Et Biophysica Acta·F M De La Vega, T Mendoza-Figueroa
Aug 1, 1989·Differentiation; Research in Biological Diversity·W Kuri-Harcuch, T Mendoza-Figueroa

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