Triglyceride-rich lipoproteins from hypertriglyceridemic subjects induce a pro-inflammatory response in the endothelium: Molecular mechanisms and gene expression studies

Journal of Molecular and Cellular Cardiology
G D NorataA L Catapano

Abstract

Triglyceride-rich lipoproteins (TGRLs) are a cardiovascular risk factor and induce endothelial dysfunction. In the present study we investigated the effects of TGRLs from type IV hyperlipidemic and normolipidemic subjects on endothelial activation focusing on the effects on intracellular pathways and gene expression. A total of 54 subjects, 30 hypertriglyceridemic (triglyceride (TG) levels 284+/-101 mg/dl) and 23 normotriglyceridemic (TG levels 109+/-40 mg/dl) were enrolled as lipoprotein donors. TGRLs were isolated from hypertriglyceridemic (H-TGRL) and normotriglyceridemic (N-TGRL) subjects. RNA from human endothelial cells incubated with N-TGRL or H-TGRL was prepared for cDNA microarray analyses. Western blotting was used to study intracellular signaling pathways. Regulated genes were further studied with real-time PCR, immunofluorescence and FACS. Furthermore, a protein/DNA array and chromatin-immunoprecipitation were used to identify transcription factors involved in the observed effects. Both N-TGRL and H-TGRL activated ERK1/2 and p38 MAPK. However, there were differences in the pattern of upregulated target genes between the two types of lipoproteins in HUVECs and/or HAECs: PAI-1, VCAM-1, ELAM-1 and MCP-1 were upregulate...Continue Reading

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Citations

Dec 21, 2010·American Journal of Physiology. Heart and Circulatory Physiology·Ying I WangAnthony G Passerini
Dec 9, 2010·European Journal of Pediatrics·Assimina Galli-TsinopoulouGeorge Varlamis
Mar 29, 2014·Current Medical Research and Opinion·Angela PirilloAlberico Luigi Catapano
Oct 18, 2011·Biochimica Et Biophysica Acta·Eric A Schwartz, Peter D Reaven
May 5, 2010·Atherosclerosis. Supplements·Jonathan Dalla-RivaCaroline P Wheeler-Jones
Oct 6, 2015·Trends in Pharmacological Sciences·Giuseppe Danilo NorataAlberico L Catapano
Aug 6, 2010·Clinical Science·Robin Altman, John C Rutledge
Jun 19, 2013·Progress in Lipid Research·Kathleen M Botham, Caroline P D Wheeler-Jones
Jul 28, 2016·Applied Physiology, Nutrition, and Metabolism = Physiologie Appliquée, Nutrition Et Métabolisme·Sébastien LacroixAnil Nigam
Mar 18, 2016·Vascular and Endovascular Surgery·Alex HelkinVivian Gahtan
Mar 13, 2013·Experimental Biology and Medicine·Mariarosaria NapolitanoElena Bravo
Jul 23, 2013·Arteriosclerosis, Thrombosis, and Vascular Biology·Hnin H AungJohn C Rutledge
Mar 17, 2018·Current Medicinal Chemistry·Johan G SchnitzlerJeffrey Kroon
Sep 6, 2014·Physical Chemistry Chemical Physics : PCCP·Idir YahiatèneJohn C Rutledge
Jul 2, 2020·Biology of Sex Differences·Charly Abi-GhanemKristen L Zuloaga
Feb 6, 2020·European Journal of Preventive Cardiology·Angela PirilloAlberico L Catapano
Oct 10, 2020·Molecular Aspects of Medicine·F BonacinaG D Norata
Jul 6, 2014·BMC Systems Biology·Hnin H AungIlias Tagkopoulos

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