Triiodothyronine and follicle-stimulating hormone, alone and additively together, stimulate production of the tissue inhibitor of metalloproteinases-1 in cultured human luteinized granulosa cells

The Journal of Clinical Endocrinology and Metabolism
S GoldmanZ Kraiem

Abstract

Thyroid disorders have been frequently associated with menstrual disturbances and impaired fertility. To characterize the nature of thyroid hormone action in the ovary, the direct effects of T3-gonadotropin interactions were investigated in vitro using a culture system of human luteinized granulosa cells in serum-free medium. Although FSH alone was devoid of any significant effect on cell proliferation, it inhibited T3-stimulated cell growth. The electrophoretic profiles of the radiolabeled proteins induced by the different hormonal treatments revealed similarity in overall protein patterns but differences in intensity of labeling. Human CG, alone or combined with T3, had no major influence on the total intensity of labeling compared with control, whereas T3 or FSH alone reduced total labeling intensity but a 30,000 Da protein band was increased. FSH combined with T3 augmented the total intensity of labeling, including the 30,000-Da protein band. Western blot analysis revealed the presence of the tissue inhibitor of metalloproteinases-1 (TIMP-1), mol wt 30,000, known to play a key role in ovarian function. TIMP-1 was dose dependently stimulated by T3 and FSH, and an additive effect was obtained when both hormones were combined....Continue Reading

Citations

Feb 6, 2004·Toxicology and Applied Pharmacology·Paul S CookeRobert E Chapin
Nov 5, 2003·European Journal of Obstetrics, Gynecology, and Reproductive Biology·Shlomit Goldman, Eliezer Shalev
Oct 6, 2005·Reproductive Biology and Endocrinology : RB&E·Mohamed F M MitwallyMichael P Diamond
Jul 11, 2019·Biology of Reproduction·Anna TetkovaJaroslav Kalous

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