Trim21 regulates Nmi-IFI35 complex-mediated inhibition of innate antiviral response

Virology
Anshuman DasAsit K Pattnaik

Abstract

In this study, using an immunoprecipitation coupled with mass spectrometry approach, we have identified the E3 ubiquitin ligase Trim21 as an interacting partner of IFI35 and Nmi. We found that this interaction leads to K63-linked ubiquitination on K22 residue of Nmi, but not IFI35. Using domain deletion analysis, we found that the interaction is mediated via the coiled-coil domain of Nmi and the carboxyl-terminal SPRY domain of Trim21. Furthermore, we show that depletion of Trim21 leads to significantly reduced interaction of Nmi with IFI35, which results in the abrogation of the negative regulatory function of the Nmi-IFI35 complex on innate antiviral signaling. Thus, Trim21 appears to be a critical regulator of the functions of the Nmi-IFI35 complex. Overall, the results presented here uncover a new mechanism of regulation of the Nmi-IFI35 complex by Trim21, which may have implications for various autoimmune diseases associated uncontrolled antiviral signaling.

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Citations

Oct 19, 2017·Nature Communications·Zhikai XiahouHuanhuan Liang
Jun 17, 2020·Genome Biology·Craig H KerrLeonard J Foster
Jan 29, 2021·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Munther Alomari
Mar 25, 2021·American Journal of Respiratory Cell and Molecular Biology·Wei OuyangFeng Xu
Aug 8, 2021·Proceedings of the National Academy of Sciences of the United States of America·Xizhong JingHuanhuan Liang

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