TRIM28 mediates primer binding site-targeted silencing of Lys1,2 tRNA-utilizing retroviruses in embryonic cells.

Proceedings of the National Academy of Sciences of the United States of America
Daniel WolfStephen P Goff

Abstract

Murine leukemia viruses (MLVs) and related retroelements are potently restricted in embryonic cells by postintegration transcriptional silencing, likely to protect the germ line from insertional mutagenesis. This silencing is in large part attributable to the presence of a nuclear repression complex, which targets a sequence element of the proviral DNA, the repressor-binding site. The repressor-binding site closely overlaps the tRNA primer binding site, a highly conserved sequence essential for virus replication and defining the site of initiation of DNA synthesis during reverse transcription. We have recently demonstrated that the cellular corepressor TRIM28 is recruited to the proline tRNA primer-binding site used by many MLVs and is required to mediate this silencing. Here, we show that TRIM28 is also required for the restriction of retroviruses using a completely distinct tRNA for the priming of their DNA synthesis, namely Lys-1,2 tRNA. These results generalize the role of TRIM28 in retroviral restriction and suggest that this system has evolved to restrict multiple retroviruses.

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Citations

Mar 10, 2009·Nature·Daniel Wolf, Stephen P Goff
Jan 8, 2010·The Journal of Biological Chemistry·Chae Young HwangByeongwoon Song
Mar 11, 2009·Molecular and Cellular Biology·Irina A MaksakovaDixie L Mager
Oct 3, 2012·PLoS Genetics·Priyanka SharmaChristian Muchardt
Nov 28, 2012·PloS One·Ignacio Marín
Jan 27, 2012·Current Opinion in Virology·Theodora Hatziioannou, Paul D Bieniasz
Jul 9, 2009·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Bo GaoSidong Xiong
Dec 14, 2011·Stem Cells·Lyle ArmstrongMiodrag Stojkovic
Mar 26, 2010·Journal of Cellular Biochemistry·Clara Y Cheong, Thomas Lufkin
Jan 18, 2013·The Journal of General Virology·Gernot WolfFinn Skou Pedersen
Jun 10, 2011·Biochemical and Biophysical Research Communications·Shanshan YuSidong Xiong
Sep 6, 2013·Current Opinion in Virology·Marie Dewannieux, Thierry Heidmann
Jun 15, 2011·Cell Host & Microbe·Awatef AllouchAnna Cereseto
May 23, 2015·Journal of Virology·Daniel O Griffin, Stephen P Goff
Jan 29, 2016·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·Tove Christensen
Mar 13, 2020·Nature Communications·Alvin YuGregory A Voth
Jul 16, 2010·The Journal of Immunology : Official Journal of the American Association of Immunologists·Bo GaoSidong Xiong
Oct 1, 2020·Viruses·Rocio Enriquez-GascaHelen M Rowe
Dec 21, 2017·The Journal of Biological Chemistry·Amulya YaparlaLeon Grayfer
Jan 22, 2011·Virology·Helen M Rowe, Didier Trono
Jun 1, 2014·Genome Research·Priscilla TurelliDidier Trono

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