TRIM29 facilitates the epithelial-to-mesenchymal transition and the progression of colorectal cancer via the activation of the Wnt/β-catenin signaling pathway.

Journal of Experimental & Clinical Cancer Research : CR
Juntao SunJie Zhong

Abstract

Tripartite Motif 29 (TRIM29) has been newly identified as being implicated in cancer progression. However, the biological role and molecular mechanism of TRIM29 in the invasion and metastasis of colorectal cancer (CRC) remain to be determined. The expression levels of TRIM29 and β-catenin in CRC patient specimens were detected by immunohistochemistry. Recombinant lentivirus vectors containing the TRIM29 gene and its small hairpin interfering RNAs were constructed and transduced into CRC cells. Wound-healing and Transwell assays were performed to evaluate the migration and invasion abilities of CRC cells in vitro. Hepatic metastasis models in nude mice were established to validate the function of TRIM29 in vivo. Moreover, the expressions of epithelial-to-mesenchymal transition (EMT)-associated proteins were detected by qRT-PCR and Western blotting in CRC cells. Finally, Western blotting, qRT-PCR, luciferase reporter assays, and immunofluorescence assays were used to explore the molecular mechanisms of TRIM29 in CRC progression. Increased TRIM29 expression positively correlated with lymph node metastasis and β-catenin expression in patient CRC tissues. Overexpression of TRIM29 promoted invasion and metastasis of CRC cells in vitr...Continue Reading

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Citations

Jun 7, 2019·Clinical and Experimental Pharmacology & Physiology·Weizhi LiShuzhen Kong
Oct 18, 2020·International Journal of Molecular Sciences·Wolfgang EberhardtJosef Pfeilschifter
Jun 29, 2021·Genes & Diseases·Juan LiuZhaohui Feng
Oct 5, 2020·Biochimica Et Biophysica Acta. Molecular Cell Research·Liang HaoHua-Qin Wang

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Methods Mentioned

BETA
dissection
reverse transcription-PCR
PCR
Protein Extraction
transfection
nuclear translocation

Software Mentioned

SPSS

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