TRIM32 promotes inflammatory responses in rheumatoid arthritis fibroblast-like synoviocytes

Scandinavian Journal of Immunology
Tian LiangLanping Xu

Abstract

Rheumatoid arthritis (RA) is a worldwide autoimmune disease. The study of its aetiology and mechanism has always been a focus topic in medicine. This research was designed to investigate the effect of E3 ubiquitin ligase tripartite motif protein 32 (TRIM32) in rheumatoid arthritis (RA). We found in fibroblast-like synoviocytes (FLS) of RA patients, the expression of TRIM32 was significantly increased compared with its expression in osteoarthritis (OA) patients FLS. A widely used pro-inflammatory stimuli tumour necrosis factor-alpha (TNF-α) was found to promote TRIM32 expression in a time-dependent manner. Furthermore, we observed that overexpression of TRIM32 aggravated the production of pro-inflammatory cytokines in FLS, silencing of TRIM32 showed the consistent results. In addition, TRIM32 was found to activate nuclear factor κB (NF-κB) signalling pathway, and TRIM32 could interact with TNF receptor-associated factor 2 (TRAF2) to promote the K63-linked polyubiquitination of TRAF2 in RA-FLS. In conclusion, we suggested that TRIM32 as a positive regulator of inflammatory responses in RA-FLS.

References

Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Oct 21, 2005·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Germana Meroni, Graciana Diez-Roux
May 30, 2006·Osteoarthritis and Cartilage·J A Roman-Blas, S A Jimenez
Aug 5, 2008·BMC Evolutionary Biology·Marco SardielloGermana Meroni
Oct 7, 2008·Nature Reviews. Immunology·Keiko OzatoHerbert C Morse
Jan 1, 2012·Advances in Experimental Medicine and Biology·Valeria CambiaghiPier Giuseppe Pelicci
Feb 24, 2015·Human Molecular Genetics·Ekaterina I MokhonovaMelissa J Spencer
Feb 24, 2016·Mediators of Inflammation·Reka KugyelkaFerenc Boldizsar
Feb 2, 2018·PLoS Pathogens·Konstantin M J Sparrer, Michaela U Gack
Aug 7, 2018·Journal of Cellular and Molecular Medicine·Changming WangQingping Cai
Mar 29, 2019·Cancer Cell International·Shengli PanXianju Qin
Sep 2, 2019·Biochemical and Biophysical Research Communications·Qiong HuangMin Xu

❮ Previous
Next ❯

Citations

Jan 14, 2021·Cell Biology International·Tingting WanYanbo Li

❮ Previous
Next ❯

Related Concepts

Related Feeds

Autoimmune Diseases

Autoimmune diseases occur as a result of an attack by the immune system on the body’s own tissues resulting in damage and dysfunction. There are different types of autoimmune diseases, in which there is a complex and unknown interaction between genetics and the environment. Discover the latest research on autoimmune diseases here.