Trimeric structure of the mouse Kupffer cell C-type lectin receptor Clec4f

FEBS Letters
Zhenlin OuyangYurong Wen

Abstract

The C-type lectin receptor Clec4f has been identified as a specific surface marker for Kupffer cells, although its ortholog is absent in humans and its biological function remains elusive. Here, we report the crystal structure of a truncated mouse trimeric Clec4f. The orientation between the carbohydrate-recognition domain of Clec4f and its neck region differs from other C-type lectins, resulting in an observed distance of 45 Å between the glycan-binding sites within the Clec4f trimer. Interestingly, the trimeric coiled-coil interface within its heptad neck region contains multiple polyglutamine interactions instead of the predominantly hydrophobic leucine zipper found in other C-type lectin receptors. The Clec4f trimeric structure displays unique features regarding its assembly and ligand recognition, shedding light on the evolution and diversity of the C-type lectin family.

References

Jan 1, 1986·Differentiation; Research in Biological Diversity·M SatoT Muramatsu
Dec 15, 1994·Structure·W I Weis, K Drickamer
Feb 19, 2002·The Journal of Biological Chemistry·Kenneth K-S NgWilliam I Weis
Apr 4, 2003·Glycobiology·Andrew J FaddenKurt Drickamer
Dec 2, 2004·Acta Crystallographica. Section D, Biological Crystallography·Paul Emsley, Kevin Cowtan
Mar 6, 2007·Nature Medicine·Lot de WitteTeunis B H Geijtenbeek
Apr 11, 2007·The Journal of Biological Chemistry·Hadar FeinbergWilliam I Weis
Apr 22, 2008·Nucleic Acids Research·A DereeperO Gascuel
Jun 17, 2008·Current Opinion in Structural Biology·Gevorg Grigoryan, Amy E Keating
Aug 1, 2007·Journal of Applied Crystallography·Airlie J McCoyRandy J Read
Feb 4, 2010·Acta Crystallographica. Section D, Biological Crystallography·Wolfgang Kabsch
Feb 4, 2010·Acta Crystallographica. Section D, Biological Crystallography·Paul D AdamsPeter H Zwart
Feb 26, 2010·The Journal of Biological Chemistry·Hadar FeinbergWilliam I Weis
Oct 29, 2010·Nature Communications·Christoph J WienkenStefan Duhr
Jul 21, 2012·Molecular Microbiology·Kristof MoonensHenri De Greve
Jul 15, 2015·Current Opinion in Structural Biology·Kurt Drickamer, Maureen E Taylor
Jan 28, 2016·Nature Communications·Charlotte L ScottMartin Guilliams
Jun 25, 2016·Molecular Imaging and Biology : MIB : the Official Publication of the Academy of Molecular Imaging·Fang ZhengAlain Beschin
Jul 19, 2017·Proceedings of the National Academy of Sciences of the United States of America·Yun LiLijun Xia
Mar 28, 2018·Nature Reviews. Immunology·Gordon D BrownLauren Whitehead
Oct 30, 2018·Journal of the American Heart Association·Yvonne Döring, Emiel P C van der Vorst

❮ Previous
Next ❯

Citations

Sep 19, 2020·Journal of Invertebrate Pathology·Tao GengShuchang Wang
Mar 12, 2021·Frontiers in Immunology·Fang ZhengGeert Raes

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.