Trimethylguanosine Synthase1 (TGS1) Is Essential for Chilling Tolerance

Plant Physiology
Jinpeng GaoJohn Browse

Abstract

Chilling stress is a major factor limiting plant development and crop productivity. Because the plant response to chilling is so complex, we are far from understanding the genes important in the response to chilling. To identify new genes important in chilling tolerance, we conducted a novel mutant screen, combining a confirmed SALK T-DNA insertion collection with traditional forward genetics. We screened a pool of more than 3700 confirmed homozygous SALK T-DNA insertion lines for visible defects under prolonged growth at 5°C. Of the chilling-sensitive mutants we observed, mutations at one locus were characterized in detail. This gene, At1g45231, encodes an Arabidopsis (Arabidopsis thaliana) trimethylguanosine synthase (TGS1), previously uncharacterized in the plant kingdom. We confirmed that Arabidopsis TGS1 is a functional ortholog of other trimethylguanosine synthases based both on its in vitro methyltransferase activity and on its ability to rescue the cold-growth inhibition of a Saccharomyces cerevisiae tgs1Δ mutant in vivo. While tgs1 mutant plants grew normally at 22°C, their vegetative and reproductive growth was severely compromised under chilling conditions. When we transgenically expressed TGS1 in the mutant plants, ...Continue Reading

Citations

Dec 19, 2019·Frontiers in Plant Science·Carolina ColonoSilvina C Pessino
Jan 23, 2018·Frontiers in Plant Science·Misato Ohtani
Jul 15, 2021·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Candy M TaylorMatthew N Nelson
Jun 10, 2021·Journal of Experimental Botany·Varvara DikayaMarkus Schmid

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