Trinitrophenylation of bovine growth hormone

International Journal of Peptide and Protein Research
M J Biscoglio de Jimenez BoninoJ A Santomé

Abstract

Bovine growth hormone was chemically modified with picryl sulfonic acid, at pH 8.4 during 2 and 5 min of reaction. The N-terminal residue provides the most reactive amino group followed by the epsilon-amino groups of lysine 179 and lysines 143, 69, 111, 170 and 166 in decreasing order. These results agree with those obtained previously with equine growth hormone, except that residue 156 is not modified in bovine growth hormone. An important decrease in biological activity occurs between 2 and 5 min of reaction without sensible modification in the alpha-helix content of the molecule.

References

Apr 30, 1977·Archives of Biochemistry and Biophysics·O CasconeJ A Santomé

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Citations

Sep 1, 1988·International Journal of Peptide and Protein Research·M R Ermácora, J L Rivero
Aug 1, 1993·The International Journal of Biochemistry·V MihajlovicM J Biscoglio de Jiménez Bonino

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