Trio, a novel high fecundity allele: I. Transcriptome analysis of granulosa cells from carriers and noncarriers of a major gene for bovine ovulation rate

Biology of Reproduction
Mamat H KamalludinBrian W Kirkpatrick

Abstract

A major gene for bovine ovulation rate has been mapped to a 1.2 Mb region of chromosome 10. Screening of coding regions of positional candidate genes within this region failed to reveal a causative polymorphism, leading to the hypothesis that the phenotype results from differences in candidate gene expression rather than alteration of gene structure. This study tested differences in expression of positional candidate genes in granulosa cells between carriers and noncarriers of the high fecundity allele, as well as characterizing differences in the transcriptomic profile between genotypes. Five carriers and five noncarriers, female descendants of "Trio," a carrier of the high fecundity allele were initially used in an RNA-seq analysis of gene expression. Four of ten samples were contaminated with theca cells, so that six samples were used in the final analysis (three of each genotype). Of 14 973 genes expressed, 143 were differentially expressed (false discovery rate P < 0.05) in carriers versus noncarriers. Among the positional candidate genes, SMAD6 was 6.6-fold overexpressed in the carriers compared to noncarriers (P < 5 × 10-5). This result was replicated in an independent group of 12 females (7 carriers and 5 noncarriers) u...Continue Reading

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Citations

May 10, 2020·Twin Research and Human Genetics : the Official Journal of the International Society for Twin Studies·Dorret I Boomsma
Jun 2, 2018·Reproduction, Fertility, and Development·Mamat H KamalludinBrian W Kirkpatrick
Oct 23, 2018·NPJ Genomic Medicine·Rahul GajbhiyeGrant W Montgomery
Apr 23, 2020·Cells·Rozenn Dalbies-TranSvetlana Uzbekova
Dec 10, 2021·Frontiers in Veterinary Science·Xiangyu WangMingxing Chu

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