TRIP13 Regulates Both the Activation and Inactivation of the Spindle-Assembly Checkpoint

Cell Reports
Hoi Tang Ma, Randy Y C Poon

Abstract

Biochemical studies have indicated that p31(comet) and TRIP13 are critical for inactivating MAD2. To address unequivocally whether p31(comet) and TRIP13 are required for mitotic exit at the cellular level, their genes were ablated either individually or together in human cells. Neither p31(comet) nor TRIP13 were absolutely required for unperturbed mitosis. MAD2 inactivation was only partially impaired in p31(comet)-deficient cells. In contrast, TRIP13-deficient cells contained MAD2 exclusively in the C-MAD2 conformation. Our results indicate that although p31(comet) enhanced TRIP13-mediated MAD2 conversion, it was not absolutely necessary for the process. Paradoxically, TRIP13-deficient cells were unable to activate the spindle-assembly checkpoint, revealing that cells lacking the ability to inactivate MAD2 were incapable in mounting a checkpoint response. These results establish a paradigm of the roles of p31(comet) and TRIP13 in both checkpoint activation and inactivation.

References

Nov 29, 2002·The EMBO Journal·Toshiyuki HabuTomohiro Matsumoto
Mar 17, 2004·Nature Structural & Molecular Biology·Xuelian LuoHongtao Yu
Jun 9, 2004·Proceedings of the National Academy of Sciences of the United States of America·Daniel R RhodesArul M Chinnaiyan
Feb 8, 2005·Current Biology : CB·Anna De AntoniAndrea Musacchio
Aug 3, 2005·Nature Reviews. Molecular Cell Biology·Phyllis I Hanson, Sidney W Whiteheart
Nov 21, 2007·Cell·Maojun YangXuelian Luo
Jul 5, 2008·Annual Review of Cell and Developmental Biology·Jillian A Pesin, Terry L Orr-Weaver
Feb 22, 2011·The Journal of Biological Chemistry·Hoi Tang Ma, Randy Y C Poon
Nov 22, 2011·Journal of Cell Science·Frederick G WesthorpeStephen S Taylor
Apr 20, 2012·The Journal of Investigative Dermatology·Marloes S van KesterCornelis P Tensen
May 1, 2012·The Journal of Biological Chemistry·Hoi Tang MaRandy Y C Poon

❮ Previous
Next ❯

Citations

May 30, 2017·Nature Genetics·Shawn YostNazneen Rahman
Jun 1, 2017·Cell Reports·Daniel Henry MarksRobert Benezra
Dec 9, 2016·American Journal of Physiology. Renal Physiology·Jeffrey D Pressly, Frank Park
May 19, 2020·Cell Cycle·Prabha SarangiAlan D D'Andrea
Jul 31, 2020·PLoS Genetics·Stefani GiacopazziNeedhi Bhalla
Jul 23, 2020·Molecular Biology of the Cell·Lénaïg DéfachellesNeedhi Bhalla
Jul 24, 2018·The Journal of Clinical Investigation·Khaled AzizJan M van Deursen
Aug 31, 2019·FEBS Letters·Daniel HaywardUlrike Gruneberg
Jan 31, 2018·Proceedings of the National Academy of Sciences of the United States of America·K L ThuD W Cescon
Jan 1, 2016·AIMS Molecular Science·Song-Tao Liu, Hang Zhang
Jun 6, 2019·F1000Research·Hiroyuki Yamano
Jan 10, 2020·Nature Cell Biology·Connor S ClairmontAlan D D'Andrea
Oct 31, 2020·Proceedings of the National Academy of Sciences of the United States of America·Kevin D Corbett
Oct 2, 2019·Journal of Pediatric Hematology/oncology·Muhammad U RashidNazneen Rahman
Mar 14, 2021·Current Genetics·Richard Cardoso da Silva, Gerben Vader
Sep 12, 2020·Current Biology : CB·Vivek B Raina, Gerben Vader
May 20, 2021·The EMBO Journal·Bas de WolfGeert J P L Kops

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cell Checkpoints & Regulators

Cell cycle checkpoints are a series of complex checkpoint mechanisms that detect DNA abnormalities and ensure that DNA replication and repair are complete before cell division. They are primarily regulated by cyclins, cyclin-dependent kinases, and the anaphase-promoting complex/cyclosome. Here is the latest research.

Bioinformatics in Biomedicine

Bioinformatics in biomedicine incorporates computer science, biology, chemistry, medicine, mathematics and statistics. Discover the latest research on bioinformatics in biomedicine here.

© 2021 Meta ULC. All rights reserved